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| Product Name | Human IL-1α ELISA KIT |
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| Description | The Eagle Biosciences Human Interleukin 1 Alpha (IL-1α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-1α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1α present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-1α is added to the wells and binds to the combination of capture antibody- IL-1α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-1α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-1α standard dilutions and IL-1α sample concentration determined. Sample Types: serum, plasma, cell culture. Range: 7.8 - 250 pg/ml. Sensitivity: 4 pg/ml. Time: 3.5 hours • The Eagle Biosciences Human Interleukin 1 Alpha (IL-1α) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 1 Alpha (IL-1α) concentrations in cell culture supernates, serum, and plasma. The Eagle Biosciences Human Interleukin 1 Alpha (IL-1α) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Interleukin 1 Alpha, IL-1α |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | L1A31-K01 |
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| Order / More Info | Human IL-1α ELISA KIT from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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