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| Product Name | Human IL-1α ELISA MAX™ Deluxe |
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| Description | IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1 binds to the cell surface type I and II IL-1 receptors (IL-1RI and IL-1RII). IL-1α, IL-1β, and IL-1RA can compete for binding to these receptors. IL-1 is a pyrogen, and it is an activating factor for lymphocytes. It also damages joints and influences liver proteins.The BioLegend ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). A human IL-1α specific mouse monoclonal antibody is first coated on a 96-well plate. Standards and samples are added to the wells, and IL-1α binds to the immobilized capture antibody. Next, a biotinylated goat polyclonal anti-human IL-1α detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of IL-1α present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader. |
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| Size | 5 Plates |
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| Concentration | ELISA |
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| Applications | n/a |
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| Other Names | Interleukin-1α, LAF, EP, LEM, MCF/MNCF, IL-1α Precoated ELISA Kit |
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| Gene, Accession, CAS # | Gene ID: 3552 |
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| Catalog # | 445804 |
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| Order / More Info | Human IL-1α ELISA MAX™ Deluxe from BIOLEGEND |
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| Product Specific References | n/a |
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