产品详情 |
Edit |   |
Antigenic Specificity | Rabbit IgG |
Clone | eB182 |
Host Species | Mouse |
Reactive Species | rabbit |
Isotype | n/a |
Format | peroxidase (HRP) conjugate |
Size | 200 µL |
Concentration | 1.0 mg/mL |
Applications | Western Blot. Rabbit IgG TrueBlot® is provided as 1000X solution. In order to conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mls). If used conservatively at 2.5mls/blot, cat. 18-8816-33 (200µL) will yield enough reagent for 80 blots.Note that there are three key procedural considerations:1. Protein A or G should not be used for the immunoprecipitation. For immunoprecipitation, anti-rat IgG beads, or anti-rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively.2. Immunoprecipitate should be completely reduced.3. Milk should be used as the blocking protein for the immunoblot. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Rabbit TrueBlot® Antibody Peroxidase Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity is observed against native Rabbit IgG by both Western blot and ELISA. |
Immunogen | n/a |
Other Names | HRP, TrueBlot, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for immunoprecipitation, TrueBlot for western blotting |
Gene, Accession # | n/a |
Catalog # | 18-8816-33 |
Price | |
Order / More Info | Rabbit IgG Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
产品资料 |
|
|