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| Antigenic Specificity | Aurora B Phospho pT232 |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human, mouse |
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| Isotype | n/a |
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| Format | immunoaffinity purified |
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| Size | 100 µg |
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| Concentration | 0.87 mg/mL |
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| Applications | ELISA, IF Microscopy, Western Blot. Phospho pT232 Aurora B antibody has been tested for use in ELISA and by western blot. See below for specific protocol. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract. HeLa cell lysate can be used as a positive control. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Anti-Phospho Aurora B pT232 affinity purified antibody is directed against the phosphorylated form of human Aurora Kinase B at the pT232 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human Aurora Kinase B pT232 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A. However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross reactivity with Aurora Kinase B from other sources is not known. |
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| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 223-234 of Human Aurora Kinase B protein. |
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| Other Names | rabbit anti-Aurora B pT232 Antibody, Phospho Aurora B, AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody |
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| Gene, Accession # | AURKB, Gene ID: 9212, NCBI: 83776600, UniProt: Q96GD4 |
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| Catalog # | 600-401-677 |
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| Price | |
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| Order / More Info | Aurora B Phospho pT232 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Nijenhuis W, Vallardi G, Teixeira A, Kops GJ, Saurin AT. (2014) Negative feedback at kinetochores underlies a responsive spindle checkpoint signal. Nat Cell Biol. 2014 Dec;16(12):1257-64. doi: 10.1038/ncb3065. Epub 2014 Nov 17. |
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