| 产品详情 |
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| Antigenic Specificity | Caspase-2, N-terminus |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | mouse, rat |
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| Isotype | n/a |
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| Format | affinity purified |
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| Size | 100 µg |
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| Concentration | 0.44 mg/mL |
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| Applications | ELISA, Western Blot. This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with 48 kDa mouse and rat caspase-2. A control and rat caspase-2 transfected human 293 cell lysate was used to show by immunoblotting that this antibody detects rat caspase-2. No reaction was observed against human caspase-2. Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 (-/-) mice (not shown) was used to show that this antibody cross reacts with mouse caspase-2.This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product. A 1:750 dilution is recommended for immunoblot. This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product. A 1:750 dilution is recommended for immunoblot. This product was assayed against 1.0 µg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and TMB as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product. A 1:750 dilution is recommended for immunoblot. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This affinity purified antibody is directed against rat and mouse caspase-2. The antibody detects long and short forms of the protein. Antiserum was purified against the immunizing peptide. This antibody reacts with mouse and rat caspase-2, but not caspase-2 from human sources. Cross reactivity with caspase-2 from other sources has not been determined. Rat and mouse caspase-2 show 94% homology. There is no significant homology to the human form. |
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| Immunogen | A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker. |
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| Other Names | rabbit anti-Caspase-2 Antibody, Apoptosis related cysteine peptidase antibody, CASP-2, CASP 2 antibody, Casp 2L Pro antibody, CASP2 antibody, Caspase 2 apoptosis related cysteine protease antibody, Caspase2 antibody, Developmentally down regulated 2 antibody, ICH 1 antibody, ICH-1, NEDD-2, NEDD2, NEDD 2 |
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| Gene, Accession # | Caspase-2, Gene ID: 64314, NCBI: AAD33684.1, UniProt: P55215 |
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| Catalog # | 600-401-412 |
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| Price | |
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| Order / More Info | Caspase-2, N-terminus Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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