| 产品详情 |
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| Antigenic Specificity | NFkB p65, internal |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human |
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| Isotype | n/a |
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| Format | purified |
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| Size | 100 µg |
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| Concentration | 1.0 mg/mL |
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| Applications | ELISA, Gel Shift, Immunohistochemistry, IF Microscopy, Western Blot. NFkB gel shift assays are assembled in 20µl reactions containing 0.28 pmoles NFkB oligo in 10mM Tris (pH 7.6), 50 mM NaCl, 0.5 mM EDTA, 1.0 mM DTT, 10% glycerol. Some procedures specify the addition of 0.05% NP-40. When using purified protein, 250-300 ng should be sufficient to produce a gel shifted complex, while 10µg HeLa nuclear extract is utilized. The gel shift reactions are then incubated at room temperature for 30 minutes. The complexes are resolved on Tris-Glycine acrylamide gels. Loading dye containing bromophenol blue and xylene cyanol should be added to the negative control reaction only, as these dyes can increase the dissociation of the NFkB complexes. When using HeLa nuclear extract as the source of binding proteins, two sequence-specific gel-shifted complexes are expected, consisting of p50/p50 homodimers and p50/p65 heterodimers. For cells expressing p52, p50, and p65, as many as four sequence-specific gel-shifted complexes could be observed (p52/p52, p50/p50, p52/p65, p50/p65), and if high levels of p65 are present, the p65/p65 homodimer may also be weakly detected. The following reagents have been observed to enhance NFkB binding in vitro: millimolar amounts of GTP and ATP, spermine, spermidine, barium or calcium ions, and µM amounts of Co+3(NH3)6. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This affinity-purified antibody is directed against the nuclear localization sequence (NLS) NLS of human p65 and is useful in determining its presence in various assays. The epitope recognized overlaps the NLS of the p65 subunit of the NFkB heterodimer. Therefore, the antibody selectively binds to the activated form of NFkB. Anti-NFkB p65 NLS may react non-specifically with other proteins. |
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| Immunogen | This antibody was purified from whole rabbit serum prepared by repeated immunizations with the NFkB p65 peptide corresponding to the NLS of the human protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. |
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| Other Names | rabbit anti-NFKB p65 antibody, rabbit anti-p65 antibody, rabbit anti-NLS specific antibody, nuclear localization sequence, NFKB, NFkβ, NF-kB, NF-kappaB, NFkappaB, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3 |
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| Gene, Accession # | RELA, Gene ID: 5970, NCBI: 223468676, UniProt: Q04206 |
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| Catalog # | 600-401-271 |
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| Price | |
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| Order / More Info | NFkB p65, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Starkey JM, Haidacher SJ, LeJeune WS, et al. (2006) Diabetes-induced activation of canonical and noncanonical nuclear factor-kappaB pathways in renal cortex. Diabetes. May;55(5):1252-1259.Khoury J, Ibla JC, Neish AS, Colgan SP. Antiinflammatory adaptation to hypoxia through adenosine-mediated cullin-1 deneddylation. J. Clin. Invest. 2007 Mar;117(3):703-711. Liu Q, Li J, Khoury J, et al. Adenosine signaling mediates SUMO-1 modification of IkappaBalpha during hypoxia and reoxygenation. J. Biol. Chem. 2009 May 15;284(20):13686-13695.Yu B, Chang J, Liu Y, Li J, Kevork K, Al-Hezaimi K, Graves DT, Park NH, Wang CY. (2014) Wnt4 signaling prevents skeletal aging and inflammation by inhibiting nuclear factor-?B. Nat Med. 2014 Sep;20(9):1009-17. doi: 10.1038/nm.3586. Epub 2014 Aug 10. Erratum in: Nat Med. 2015 Sep;21(9):1101. |
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