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Antigenic Specificity | NFkB p65 phospho S276 |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human |
Isotype | n/a |
Format | delipidated & defibrinated |
Size | 100 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, Western Blot. This phospho specific polyclonal antibody reacts human pS276 p65 and shows minimal reactivity by western blot with non-phosphorylated p65 and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a 1:1,000 dilution is recommended. A 65 kDa band corresponding to human p65 is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This product was prepared from monospecific antiserum by delipidation and defibrination. This phospho specific polyclonal antibody is specific for phosphorylated pS276 human p65. Reactivity with non-phosphorylated p65 is minimal. Cross reactivity with pS276 phosphorylated p65 from mouse, rat or other species has not been determined. |
Immunogen | NFkB p65 (Rel A) peptide corresponding to an internal region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
Other Names | rabbit anti-NFKB p65 pS276 antibody, rabbit anti-p65 antibody, rabbit anti-RelA antibody, NFKB, NFkβ, NF-kB, NF-kappaB, NFkappaB, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3 |
Gene, Accession # | RELA, Gene ID: 5970, NCBI: 223468676, UniProt: Q04206 |
Catalog # | 600-401-264 |
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Order / More Info | NFkB p65 phospho S276 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, and Aggarwal BB (2002) Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. J Immunol 169-11: 6490-7.Kawakami A, Osaka M, Aikawa M, et al. (2008) Toll-like receptor 2 mediates apolipoprotein CIII-induced monocyte activation. Circ. Res. Dec 5;103(12):1402-1409. |
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