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Antigenic Specificity | NFkB p65 (RelA) Phospho S276, internal |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human |
Isotype | n/a |
Format | delipidated & defibrinated |
Size | 100 µL |
Concentration | 80 mg/mL |
Applications | ELISA, Immunohistochemistry, Western Blot. Phospho NFkB antibody reacts human pS276 p65 and shows minimal reactivity by western blot with non-phosphorylated p65 and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. A 1:500 dilution has been used for staining p65 in human kidney tissue by IHC. Tissue was formalin fixed and paraffin embedded. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. All conditions must be user optimized. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-NFkB p65 (RelA) Phospho S276 Antibody was prepared from monospecific antiserum by delipidation and defibrination. This phospho specific polyclonal antibody is specific for phosphorylated pS276 human p65. Reactivity with non-phosphorylated p65 is minimal. Cross reactivity with pS276 phosphorylated p65 from mouse, rat or other species has not been determined. |
Immunogen | NFkB p65 (Rel A) peptide corresponding to an internal region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
Other Names | rabbit anti-NFkB p65 pS276 Antibody, rabbit anti-RelA pS276 Antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Anti-NF-kB antibody |
Gene, Accession # | RELA, Gene ID: 5970, NCBI: 223468676, UniProt: Q04206 |
Catalog # | 100-401-264 |
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Order / More Info | NFkB p65 (RelA) Phospho S276, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, and Aggarwal BB (2002) Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. J Immunol 169-11: 6490-7.Spooren A, Kolmus K, Vermeulen L, et al. (2010) Hunting for serine 276-phosphorylated p65. J. Biomed. Biotechnol. ;2010:275892.Begley LA, Kasina S, Mehra R, et al. (2008) CXCL5 promotes prostate cancer progression. Neoplasia. 2008 Mar;10(3):244-254. |
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