| 产品详情 |
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| Antigenic Specificity | Alkaline Phosphatase |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | E. coli |
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| Isotype | n/a |
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| Format | IgG fraction |
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| Size | 25 µL |
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| Concentration | 1.0 mg/ml |
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| Applications | ELISA, Western Blot. ALKALINE PHOSPHATASE Antibody has been assayed against 1.0 ug of Alkaline Phosphatase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:25,000 to 1:125,000 of the reconstitution concentration is suggested for this product. A 1:10,000 dilution has been reported to be useful for the detection by immunoblot of an integral membrane protein from a gram-positive organism expressed as a LacZ/PhoA chimeric derivative in E.coli. The researcher should determine optimal titers for other applications. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Anti-ALKALINE PHOSPHATASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined. Some minimal crossreactivity has been reported when lysates of cell extracts overproducing E.coli proteins are probed. |
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| Immunogen | Alkaline Phosphatase [E.coli] |
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| Other Names | rabbit anti-Alkaline Phosphatase Antibody, Akp2 antibody, ALP antibody, Nagao isozyme antibody, Placental alkaline phosphatase antibody, Placental like alkaline phosphatase antibody, PLAP 1 antibody, PLAP like antibody, Regan isozyme antibody, Kasahara isozyme antibody |
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| Gene, Accession # | phoA, Gene ID: 945041, NCBI: P00634.1, UniProt: P00634 |
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| Catalog # | 200-4134S |
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| Price | |
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| Order / More Info | Alkaline Phosphatase Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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