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| Product Name | CellCountEZ™ Cell Survival Assay Kit |
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| Description | CellCountEZ™ Cell Survival Assay Kit is a tissue culture media-based assay that can measure metabolically active live cells and quantify cell death caused by radiation, chemotherapeutics or toxins. CellCountEZ™ is based on the ability of mammalian cells to rapidly and efficiently convert hydroxyethyl disulfide (HEDS) into mercaptoethanol (ME) through a bioreduction mechanism. Bioconversion of HEDS to ME relies on the activity of the oxidative pentose phosphate cycle (OPPC). CellCountEZ™ measures ME in the extracellular medium without the need for cellular lysis and extraction methods, this preserves the ability to perform other cellular tests in the same culture system. CellCountEZ™ is readily soluble, membrane permeable and converted by live cells intracellularly before transport into the extracellular culture media. CellCountEZ™ offers many advantages that make it superior to common existing methods for quantifying cell growth and survival. |
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| Size | 1 kit |
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| Concentration | n/a |
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| Applications | CellCountEZ™ Cell Survival Assay Kit contains enough reagents for 1000 assays using a 96-well plate. This assay gives a linear response for cells (0; 1,000; 5,000; 10,000; 20,000; 40,000) plated in 100 µl growth medium with up to 15% fetal bovine serum in a 96-well plate and measured 20 hours after plating. Drug toxicity could be measured for up to 4 days after treatment with drugs using this assay for 5,000 cells plated in 100 µl growth medium with up to 15% fetal bovine serum in a 96-well plate. |
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| Other Names | Toxicity, Proliferation, and Survival Assay, cell proliferation and survival, drug screening, cell viability, cell survival, cellular viability, cellular survival, HEDS assay. |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | KLD-001 |
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| Order / More Info | CellCountEZ™ Cell Survival Assay Kit from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Li J, Zhang D, Ward KM, Prendergast GC, and Ayene IS. (2012) Hydroxyethyl disulfide as an efficient metabolic assay for cell viability in vitro. Toxicol. In Vitro Jun;26(4):603-12.Ayene IS, Biaglow JE, Kachur AV, Stamato TD and Koch CJ. (2008) Mutation in G6PD gene leads to loss of cellular control of protein glutathionylation: Mechanism and Implication. J. Cell Biochem. 103: 123-135.Ayene IS, Stamato TD, Mauldin SK, Biaglow JE, Tuttle SW, Jenkins SF and Koch CJ (2002) Mutation in the glucose-6-phosphate dehydrogenase gene leads to inactivation of Ku DNA end binding during oxidative stress. J. Biol. Chem. 277: 9929-9935. |
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