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| Product Name | Rabbit TrueBlot® Set (IP Agarose beads) |
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| Description | Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated anti-rabbit IgG immunoblotting (second step) reagent. Rabbit IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Western Blot data with Rabbit IgG TrueBlot®, simply substitute the conventional HRP anti-rabbit IgG blotting reagent with Rabbit IgG TrueBlot® and follow the prescribed protocol for sample preparation and immunoblotting.Rabbit IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitatingantibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Rabbit IgG TrueBlot may also be used for detection in immunoblotting assays that do not employ immunoprecipitation. |
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| Size | 1 set |
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| Concentration | n/a |
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| Applications | Immunoprecipitation, Western Blot. Rabbit IgG TrueBlot® is provided as 1000X solution. In order to conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 mls). If used conservatively at 2.5mls/blot, cat. 18-8816-31 (50µL) will yield enough reagent for 20 blots.Note that there are three key procedural considerations:1. Protein A or G should not be used for the immunoprecipitation. For immunoprecipitation, anti-rat IgG beads, or anti-rabbit IgG beads should be used for rat or rabbit immunoprecipitating antibodies, respectively.2. Immunoprecipitate should be completely reduced.3. Milk should be used as the blocking protein for the immunoblot.Components:1. Rabbit IgG TrueBlot®. An HRP-conjugated second step reagent reacting with rabbit IgGs for optimal signal detection in immunoprecipitation/immunoblotting experiments.2. Anti-Rabbit Ig IP Beads: 2.5 ml. Binds 1 mg Ig/ml beads.3. Western blot incubation traySpecial Notes:Upon initial use of the IP beads, we recommend that the vial be inverted several times to get the beads into suspension. We recommend to use a large bore pipet to pipet up the liquid for use.For storage of the opened vial of beads, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer. |
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| Other Names | Anti-Rabbit Immunoglobulin Gamma, Agarose-conjugated IgG, Gt-a-Rb IgG, Goat-anti-Rabbit IgG, TrueBlot for immunoprecipitation, IP Agarose beads for TrueBlot, HRP, HRP TrueBlot ULTRA, Peroxidase TrueBlot, TrueBlot for IP/WB, TrueBlot for western blotting |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 88-1688-31 |
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| Order / More Info | Rabbit TrueBlot® Set (IP Agarose beads) from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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