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| Product Name | Blotto B Pre-Mixed |
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| Description | This product contains highly purified immunoanalytical grade non-fat dry milk and bovine serum albumin (protease and immunoglobulin-free BSA) dissolved in a solution of Tris buffered saline. |
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| Size | 500 mL |
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| Concentration | 1X |
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| Applications | ELISA, Western Blot. Use Rockland's BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available. BLOTTO A is a convenient general purpose blocking agent. BLOTTO B is recommended for blocking when phospho specific antibodies are used. BLOTTO A and BLOTTO B provide Tween-20 in a separate vial in liquid form (p/n TW0025). Users should consider the addition of Tween-20, protease inhibitors and phosphatase inhibitors when required. Typically 50 mM NaF can be added to BLOTTO solutions to inhibit phosphatase activity. We recommend using 1X working solutions of BLOTTO A or BLOTTO B directly for blocking purposes and primary antibody dilution and incubation. |
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| Other Names | Blotto B Pre-Mixed, TBS, 1% non-fat dry milk, 1% BSA, 0.05% TWEEN-20, blocking reagents for western or ELISA |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | B553-0500 |
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| Price | |
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| Order / More Info | Blotto B Pre-Mixed from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | n/a |
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