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Product Name | Human GLP-2 ELISA Assay Kit |
Description | This Human GLP-2 ELISA Assay kit for determination of human GLP-2 in plasma and serum samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to human GLP-2 and biotin-avidin affinity system. To the wells of the plate coated with goat anti rabbit IgG, standard Antigens or samples, biotinylated human GLP-2, and rabbit anti GLP-2 antibody are added for competitive immunoreaction. After incubation and plate washing, horse?radish peroxidase (HRP) labeled streptoavidin (SA) is added to form HRP labeled SA - biotinylated GLP-2 - antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by o-phenylenediamine dihydrochloride (OPD) and the concentration of human GLP-2 is calculated. Sample Types: serum, plasma. Range: 0.412 - 100 ng/mL. Time: 20 hours • Proglucagon gene is expressed in both pancreatic A cell and intestinal L cell. Tissue-specific posttranslational processing of proglucagon by prohormone convertase produces different proglucagon derived peptides (PGDPs) in both pancreas and intestine. The most notable pancreatic PGDP is glucagon, whereas intestinal L cell produces several structurally related peptides, including glucagon-like peptide 1 (GLP-1) and 2 (GLP-2), as well as glicentin and oxyntomodulin which contain glucagon sequence in their molecules. Among PGDPs, GLP-2 has been found to show intestinal epithelial proliferation. |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Human GLP-2 ELISA Assay Kit |
Gene, Accession, CAS # | n/a |
Catalog # | YK141 |
Price | |
Order / More Info | Human GLP-2 ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |
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