| 产品详情 |
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| Antigenic Specificity | GFP |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | n/a |
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| Isotype | n/a |
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| Format | immunoaffinity purified |
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| Size | 25 µL |
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| Concentration | 1.0mg/mL |
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| Applications | ELISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot. Anti-GFP antibody is designed to detect GFP and its variants. GFP antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with Rockland's monoclonal anti-GFP (600-301-215) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots.Optimal titers for applications should be determined by the researcher. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Anti-GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins. |
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| Immunogen | The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. |
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| Other Names | rabbit anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish |
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| Gene, Accession # | n/a |
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| Catalog # | 600-401-215S |
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| Price | |
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| Order / More Info | GFP Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Jin, H. and Zangar, R. C. 2012. High-Throughput, Multiplexed Analysis of 3-Nitrotyrosine in Individual Proteins. Current Protocols in Toxicology. 51:17.15.1-17.15.16.Kwan W-H, Navarro-Sanchez E, Dumortier H, et al. Dermal-type macrophages expressing CD209/DC-SIGN show inherent resistance to dengue virus growth. PLoS Negl Trop Dis. 2008;2(10):e311. Papadopoulos C, Arato K, Lilienthal E, et al. Splice variants of the dual specificity tyrosine phosphorylation-regulated kinase 4 (DYRK4) differ in their subcellular localization and catalytic activity. J. Biol. Chem. 2011 Feb 18;286(7):5494-5505. Rizzolio F, Lucchetti C, Caligiuri I, et al. Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1. Cell Death and Differentiation. 2012 Feb 10;Schmidt FI, Bleck CKE, Helenius A, Mercer J. Vaccinia extracellular virions enter cells by macropinocytosis and acid-activated membrane rupture. EMBO J. 2011 Aug 31;30(17):3647-3661. Torres J, Funk HM, Zegers MMP, ter Beest MBA. The syntaxin 4 N terminus regulates its basolateral targeting by munc18c-dependent and -independent mechanisms. J. Biol. Chem. 2011 Mar 25;286(12):10834-10846. Venters SJ, Cuenca PD, Hyer J. Retinal and anterior eye compartments derive from a common progenitor pool in the avian optic cup. Mol. Vis. 2011;17:3347-3363.Bleckert A, Parker ED, Kang Y, Pancaroglu R, Soto F, Lewis R, Craig AM, Wong RO. (2013) Spatial relationships between GABAergic and glutamatergic synapses on the dendrites of distinct types of mouse retinal ganglion cells across development. PLoS One. 2013 Jul 26;8(7):e69612. doi: 10.1371/journal.pone.0069612.He M. (2012) miRNA Tagging and Affinity-purification (miRAP). Bio Protoc. 2012 Oct 5;2(19). pii: e265. |
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