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| Product Name | Tocilizumab (Actemra ®) ELISA Assay Kit |
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| Description | This ELISA is based on a Tocilizumab-specific monoclonal antibody (catcher Ab, ImmunoGuide clone IG-7C84). Diluted standards and diluted samples are incubated in the microtiter plate coated with IG-7C84 mAb. After incubation, the wells are washed. A horseradish peroxidase (HRP)-conjugated anti-Tocilizumab antibody is added and binds to the Tocilizumab. Following incubation, the wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of Tocilizumab in the sample or standard. Results of samples can be determined by using the standard curve. Binding of Tocilizumab to the solid phase, pre-coated with IG-7C84, is inhibited by recombinant human interleukin-6 receptor-α (rh-IL6Rα) in a concentration dependent manner. Therefore, the ImmunoGuide Tocilizumab ELISA (mAb-Based) measures the free form of Tocilizumab. Sample Types: serum, plasma. Range: 6 - 200 ng/mL. Sensitivity: 2 ng/mL. Time: 2.5 hours • The drug Tocilizumab (trade name Actemra®) is a humanized monoclonal antibody (mAb) that specifically targets both soluble interleukin-6 receptor (sIL-6R) and membrane bound interleukin-6 receptor (mIL-6R) with high affinity, thereby preventing pro-inflammatory effects of IL-6. The specificity of this testsystem is achieved by using a monoclonal antibody (clon IG-7C84) for the coating of the microtiter plate and also as a conjugate. This Eagle Biosciences Tocilizumab ELISA (mAb-based) is developed for the specific measurement of Tocilizumab in sera, plasma and other biological fluids by the advantage of using a site-directed IG-7C84 mouse monoclonal antibody (mAb) specific for Tocilizumab only. Binding of Tocilizumab to the solid phase, pre-coated with IG-7C84, is inhibited by recombinant human interleukin-6 receptor alpha (rh-IL6Ra) in a concentration dependent manner. Therefore, this Tocilizumab ELISA (mAb-Based) measures the free form of Tocilizumab. The choice of specifically measuring the free form allows investigators to analyze the concentration-effect relationship. Due to the use of a mAb as catcher and also as detection reagent this testkit has an unmatched specificty. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Actemra, anti-drug antibody kits, anti drug shikari shakari Actemra, RoActemra, Ab, Antibodies, Antibody, ELISA Assay Kit EIA, Immunoassay, Tocilizumab, IG-AB108, Tani, Atlizumab |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | IG-AB108 |
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| Order / More Info | Tocilizumab (Actemra ®) ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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