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| Product Name | Myeloperoxidase Assay Kit (red fluorescence) |
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| Description | Amplite™ Fluorimetric Myeloperoxidase Assay Kit *Red Fluorescence*. Myeloperoxidase (MPO), most abundantly present in neutrophils and monocytes, is a green hemoprotein having peroxidase activity. It catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates; and plays an important role in the oxygen-dependent killing of tumor cells and microorganisms. MPO deficiency is a hereditary deficiency of the enzyme, which predisposes to immune deficiency. There are considerable interests in the development of therapeutic MPO inhibitors. Our Amplite™ Myeloperoxidase Assay Kit provides a quick and sensitive method for the measurement of myeloperoxidase in solution and in cell lysates. It can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The kit uses our Amplite™ Red substrate which enables a dual recordable mode. The signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm. With the Amplite™ Myeloperoxidase Assay Kit, we have detected as little as 0.1 mU/ml myeloperoxidase in a 100 µL reaction volume. The kit can be automated for high throughput screenings of MPO inhibitors. |
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| Size | 200 tests |
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| Concentration | n/a |
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| Applications | Biossay, Fluorometric |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 11301 |
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| Order / More Info | Myeloperoxidase Assay Kit (red fluorescence) from AAT BIOQUEST, INC. |
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| Product Specific References | n/a |
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