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Antigenic Specificity | IL-1 Receptor Type I |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, primate |
Isotype | n/a |
Format | serum |
Size | 1 mL |
Concentration | 84 mg/mL |
Applications | ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot. This antiserum against anti-Human IL-1 Receptor Type I (IL-1RI) has been tested for use in ELISA, radioimmunoassays and immunoprecipitation. Reactivity in other immunoassays is unknown.This product has been assayed for the ability to immunoprecipitate antigen. A dilution range of 1:400 to 1:800 is suggested for this immunoassay. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. This product has been assayed by ELISA against soluble (extracellular) IL-1RI using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:1,000 to 1:2000 is suggested for this product. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8,000 is suggested for this immunoassay. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This antiserum has been heated to 56°C for 30 minutes. The antiserum is directed against the extracellular domain of the human IL-1R type I, but precipitates the intact receptor expressed on human cells. In general, this antibody also detects primate IL-1RI. The antibody does not recognize human IL-1, IL-1Ra or IL-1R type II. In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the IL-1R type I (also called soluble IL-1R type I or ''decoy'' receptor) found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid. |
Immunogen | IL-1 Receptor Type I Antibody was prepared by repeated immunizations with recombinant human IL-1R type I. |
Other Names | IL1RT1 antibody, Interleukin 1 receptor alpha type 1 antibody, Interleukin 1 receptor antagonist protein (IRAP) antibody, Antigen CD121a antibody, CD121A antibody, CD121a antigen antibody, D2S1473 antibody, IL 1 antibody, rabbit anti-IL-1 Receptor Type I Antibody, rabbit anti-interleukin-1 Receptor Type I Antibody |
Gene, Accession # | IL1R1, Gene ID: 3554, NCBI: P14778.1, UniProt: P14778 |
Catalog # | 109-401-304 |
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Order / More Info | IL-1 Receptor Type I Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
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