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Antigenic Specificity | beta Actin, internal |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | mouse, rat, human, monkey, sheep, bovine, goat, guinea pig, swine, hamster |
Isotype | n/a |
Format | purified |
Size | 200 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, IF Microscopy, Western Blot. Anti-beta Actin Antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-beta-Actin Loading Control Antibody reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control. |
Immunogen | beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 359-368 of Human beta Actin. |
Other Names | rabbit anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, β actin, anti-beta actin antibody |
Gene, Accession # | ACTB, NCBI: 4501885, UniProt: P60709 |
Catalog # | 600-401-886 |
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Order / More Info | beta Actin, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | Hopkins MM, Feng X, Liu M, Parker LP, Koh DW. (2015) Inhibition of the transient receptor potential melastatin-2 channel causes increased DNA damage and decreased proliferation in breast adenocarcinoma cells. Int J Oncol. 2015 May;46(5):2267-76. doi: 10.3892/ijo.2015.2919. Epub 2015 Mar 6.Khoh-Reiter S, Jessen BA. (2009) Evaluation of the cytotoxic effects of ophthalmic solutions containing benzalkonium chloride on corneal epithelium using an organotypic 3-D model. BMC Ophthalmol. 2009 Jul 28;9:5. doi: 10.1186/1471-2415-9-5. |
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