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Antigenic Specificity | Cdc2 p34 |
Clone | POH-1 |
Host Species | Mouse |
Reactive Species | human, mouse, rat |
Isotype | IgG2a kappa |
Format | Protein A purified |
Size | 100 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, Immunoprecipitation, Western Blot. This antibody is suitable for immunohistochemistry immunoprecipitation (as active kinase), and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products. HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting. LEP fibroblast cell lysate is suggested as a negative control. Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1% saponin treatment or other permeabilization method. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | This protein A purified mouse monoclonal antibody reacts specifically with p34 cdc2 in human tissues and cell lines. This antibody is not cross reactive with other cyclin dependent kinases. Cross reactivity with p34 cdc2 from other sources, especially mouse and rat will occur. This reagent has broad interspecies reactivity. |
Immunogen | This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein. |
Other Names | mouse anti-cdc2 p34 antibody, mouse anti-cdc2 antibody, mouse anti-p34 antibody, Cell Divsion Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, DKFZp686L20222 antibody, MGC111195 antibody, p34 Cdk1 antibody, p34 protein kinase antibody |
Gene, Accession # | CDC2, Gene ID: 983, NCBI: P06493.2, UniProt: P06493 |
Catalog # | 200-301-160 |
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Order / More Info | Cdc2 p34 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | Lukas, J. et al. (1992) Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur. J. Biochem., 207, 169-176.Ye, X.S. et al. (1997) Proteolysis and tyrosine phosphorylation of p34cdc2/cyclin B. The role of MCM2 and initiation of DNA replication to allow tyrosine phosphorylation of p34cdc2. J. Biol Chem 272(52):33384-93.Palmer, A. et al. (1998) A link between MAP kinase and p34cdc2/cyclin B during oocyte maturation: p90rsk phosphorylates the p34cdc2 inhibitory kinase Myt1. EMBO J. 17, 5037-5047. |
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