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Antigenic Specificity | Mouse IL-27/P28 |
Clone | 3H12.F10 |
Host Species | Rat |
Reactive Species | mouse |
Isotype | IgG2a kappa |
Format | ion exchange purified |
Size | 25 µL |
Concentration | 1.0 mg/mL |
Applications | ELISA, Flow Cytometry, Western Blot. IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in Flow Cytometry, ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26,411 Da in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-IL-27 / p28 antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse. Cross-reactivity with IL-27 from other sources has not been determined. |
Immunogen | This Protein A purified monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E.coli) followed by hybridoma development. |
Other Names | rat anti-IL-27/p28 antibody, rat anti-interleukin 27a antibody, Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A cytokine, IL27-A, p28, IL-30 |
Gene, Accession # | IL27, Gene ID: 246779, NCBI: NP_663611.1, UniProt: Q8K3I6 |
Catalog # | 210-501-B54S |
Price | |
Order / More Info | Mouse IL-27/P28 Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
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