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| Product Name | D-Lactate Assay Kit |
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| Description | D(−)-Lactate is a stereoisomer of lactate and is present in blood at only 1-5% the concentration of L(+)-lactate. Exogenous sources of D-lactate include fermented foods such as yogurt, sauerkraut, and pickles. D-lactate is normally produced in the gastrointestinal tract, mainly by lactobacilli and bifidobacteria or is produced in the cytosol of cells by the glyoxylase pathway. Cayman's D-Lactate Assay provides a fluorescence-based method for detecting D-lactate in biological samples such as serum, plasma, blood, urine, and saliva. It can also be utilized to determine intracellular and extracellular lactate concentrations in cell culture samples. In the assay, D-lactate dehydrogenase catalyzes the oxidation of D-lactate to pyruvate, along with the concomitant reduction of NAD+ to NADH. NADH reacts with the fluorescent substrate to yield a highly fluorescent product. The fluorescent product is analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm. |
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| Size | 96 well |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | kits assays measures measurements D(-)-Lactates stereoisomers blood exogenous fermented foods yogurt sauerkraut pickles gastrointestinal tract lactobacilli bifidobacteria cytosols cells glyoxylase I II pathways methylglyoxal glutathione S-D-lactoylglutathione L-lactate L(+)-lactate pyruvates dehydrogenases LDH liver D-a-hydroxy acid D-alpha-hydroxy D-α-hydroxy mammals enzymes humans murines mitochondrial mitochondria D-lactic acidosis metabolic serum neurotoxic fermentation carbohydrates kidneys short bowel syndromes jejunoileal bypass surgery diabetes infection ischemia trauma monogastric malabsorptive nutrient sepsis plasma urine saliva intracellular extracellular cell culture samples oxidation reduction NAD+ NADH |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 700520-96WELLS |
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| Order / More Info | D-Lactate Assay Kit from CAYMAN CHEMICAL COMPANY |
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| Product Specific References | n/a |
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