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| Product Name | PROLACTIN ELISA |
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| Description | The essential reagents required for an immunoenzymatic assay include high affinity and specificity antibodies (enzyme and immobilised) with different and distinct epitope recognition, in excess, and native Antigens. In this Prolactin ELISA Assay Kit, the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal anti-PRL antibody. Upon mixing monoclonal biotinylated antibody, the enzyme labeled antibody and a serum containing the native Antigens, a reaction results between the native Antigens and the antibodies without competition or steric hindrance to form a soluble sandwich complex. The interaction is illustrated by the following equation: Ka EnzAb(p) + AgPRL + BtnAb(m) ? EnzAb(p)- AgPRL- BtnAb(m) K-a BtnAb(m) = Biotinylated Monoclonal Antibody (Excess quantity) AgPRL = Native PRL Antigens (variable quantity) EnzAb(p) = Enzyme labeled policlonal antibody (Excess quantity) EnzAb(p)- AgPRL- BtnAb(m) = Antigens-Antibodies Sandwich complex Ka = Rate constant of association K-a = Rate constant of disassociation Simultaneously the complex is deposited to the well through the high affinity reaction of streptavidin and biotinylated antibody. This interaction is illustrated below: EnzAb(p)-AgPRL-BtnAb(m) + Streptavidincw ?Immobilized complex Streptavidincw = Streptavidin immobilized on well Immobilized complex = Antibodies-Antigens sandwich bound. After equilibrium is attained, the antibody-bound fraction is separated from unbound Antigens by decantation or aspiration. The enzyme activity in the antibody-bound fraction is directly proportional to the native Antigens concentration. By using several different serum references of known Antigens values, a dose response curve can be generated from which the Antigens concentration of an unknown can be ascertained. Sample Types: serum. Range: 5 -100 ng/ml. Sensitivity: 0.12 ng/ml. Time: 1.5 hours • Prolactin is a polypeptide hormone synthesized and secreted by the Adenohypophysis (anterior Pituitary gland) and the placenta. It is also produced in other tissues including the breast and the decidua. Pituitary prolactin secretion is regulated by neuroEndocrinology neurons in the hypothalamus, most importantly by neurosecretory dopamine neurons of the arcuate nucleus, which inhibit prolactin secretion. Prolactin is present in several body fluids, including blood plasma, amniotic fluid, milk, mucosal secretions and cerebrospinal fluid. Prolactin has many effects, the most important of which is to stimulate the mammary glands to produce milk (lactation). Other possible functions of prolactin include the surfactant synthesis of the fetal lungs at the end of the pregnancy and immune tolerance of the foetus by the maternal organism during pregnancy. Prolactin may also have inhibitory effects on gonadal function when present in high concentrations. There is a diurnal cycle in prolactin secretion. During pregnancy, high circulating concentrations of estrogen promote prolactin production. The resulting high levels of prolactin secretion cause maturation of the mammary glands, preparing them for lactation. After childbirth, prolactin levels fall as the internal stimulus for them is removed. High prolactin levels also tend to suppress the ovulatory cycle by inhibiting the secretion of both FSH and GnRH. Prolactin levels may be checked as part of a sex hormone workup, as elevated prolactin secretion can suppress the secretion of FSH and GnRH, leading to hypogonadism, and sometimes causing erectile dysfunction in men. Elevations in plasma prolactin concentrations occur during ovulation, pregnancy, nursing and stress. Abnormal elevations in plasma prolactin levels (hyperprolactinemia) can occur as a result of pituitary adenomas, other anatomic and traumatic abnormalities, in response to certain pharmacologic agents and in hypothyroidism. Hypoprolactinemia (low prolactin levels) are observed in cases of hypopituitarism. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Prolactin, steroid hormone |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | DCM011 |
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| Order / More Info | PROLACTIN ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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