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| Product Name | TRAb clone (TSH Receptor Autoantibodies) ELISA |
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| Description | TRAb clone (TSH Receptor Autoantibodies) ELISA Assay Kit is a competitive enzyme immunoassay with a step by step incubation. During the first incubation the TSH receptor antibodies of the samples and calibrators bind to the receptor immobilized on the solid phase of the microtiter plate. Following an incubation period of 120 min, antibodies not bound are separated from the solid-phase immune complexes. In a second incubation step of 25 min the M22 stimulating monoclonal human antibody biotin complex binds to the free epitopes of the receptor and / or is going into competition with the autoantibodies bound. The absence of autoantibodies against TSH receptor results in a com plete saturation of the provided receptor by the M22 complex. Thus, the more autoantibodies (TRAb) are present in the sample, the less complex is bound by the TSH receptor immobilized. The bound receptor-M22 antibody complexes then react specifically with streptavidin peroxidase (SA-POD) conjugate. After the incubation period of 20 min, excessive conjugate is separated from the solid-phase immune complexes by the following washing step. Horseradish peroxidase converts the colorless substrate solution of 3,3',5,5'-tetramethylbenzidine (TMB) added into a blue product. This enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 30 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is indirectly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the concentrations of the antibodies of the standards (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve. Sample Types: serum. Range: Cut-off. Sensitivity: 0.1 IU/L. Time: 3.5 hours • TRAb clone (TSH Receptor Autoantibodies) ELISA Assay Kit is used for the quantitative determination of Thyrotropin (TSH) receptor autoantibodies in human serum. Whereas thyrotoxicosis is caused by thyroid autonomy, hyperthyroidism of Graves' disease is due to TSH receptor autoantibodies (TRAb). These autoantibodies mimic TSH effects on the thyroid cell and thus increase blood levels of T4 and T3. Consequently, the measurement of TRAb is valuable for the differential diagnosis of hyperthyroidism as well as for the follow-up of Graves' disease, both during and after its treatment by antithyroid drugs, 131-radioiodine or surgery. Possible additional diagnostic relevance has to be established in the future. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Thyrotropin (TSH) receptor autoantibodies, TRAb, T.R.A. |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | TRB31-K01 |
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| Order / More Info | TRAb clone (TSH Receptor Autoantibodies) ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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