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Product Name | Protein Expression Media, Turbo Broth™ (Liquid) pEX™ |
Description | Turbo Broth™ and Turbo Prime Broth™ are proprietary media formulations used for the cultivation of recombinant strains of E. coli. Turbo Prime Broth™ is made without animal-derived raw materials and is APF Certified. These media have been developed to improve the yield of recombinant proteins. The carbon source is glycerol which is not metabolized into acetic acid. It has a rich nutrient base which provides amino acids, vitamins, inorganic and trace minerals at levels higher than those of LB Broth. The medium is buffered at pH 7.2±0.2 with potassium phosphate to prevent a change in pH and to provide a source of phosphate. These modifications permit higher cell yields than can be achieved with LB. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 4 to 5 times that of LB Broth in shake flask cultures depending on the characteristics of the strain. Plasmid copy numbers are higher, which can als |
Size | 500ml |
Concentration | n/a |
Applications | n/a |
Other Names | n/a |
Gene, Accession, CAS # | n/a |
Catalog # | P9102-40A1 |
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Order / More Info | Protein Expression Media, Turbo Broth™ (Liquid) pEX™ from UNITED STATES BIOLOGICAL |
Product Specific References | n/a |
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