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1、FX-5000T细胞应力加载系统(flexer-cell FX5000 Tension system)
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| 1）该系统对二维、三维细胞和组织轴向和圆周应力加载； |
| 2）基于柔性膜基底变形、受力均匀;
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| 3）可实时观察细胞、组织在应力作用下的反应;
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| 4）可有选择性地封阻对细胞的应力加载;
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| 5）同时兼备细胞压力加载功能;
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| 6）多达4通道，可4个不同程序同时运行；
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| 7）同一程序中可以运行多种频率，多种振幅和多种波形；
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| 8）更好地控制在超低或超高应力下的波形；
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| 9）多种波形种类：静态波形、正旋波形、心动波形、三角波形、矩形以及各种te制波形；
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| 10)电脑系统对牵张拉伸力加载周期、大小、频率、持续时间精确智能调控
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| 应用范围：加载分析各种细胞在应力刺激下的生物化学反应，
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| 例如：骨骼细胞、肺细胞、心肌细胞、血细胞、皮肤细胞、肌腱细胞、韧带细胞、软骨细胞和骨细胞、
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| 肾膀胱细胞、平滑肌细胞/尿路上皮及尿路上皮细胞、眼上皮细胞、
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| 眼小梁组织细胞、肾小管上皮细胞、肠上皮细胞、胃上皮细胞等细胞牵张拉伸力加载 |
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2、FX-5000C细胞压力加载系统(flexer-cell FX5000 Compression system)
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| 1）该系统对各种组织、三维细胞培养物提供周期性或静态的压力加载； |
| 2）基于柔性膜基底变形、受力均匀; |
| 3）可实时观察细胞、组织在压力作用下的反应; |
| 4）可有选择性地封阻对细胞的应力加载; |
| 5）同时兼备细胞牵张力加载功能; |
| 6）多达4通道，可4个不同程序同时运行； |
| 7）同一程序中可以运行多种频率(0.01- 5 Hz)，多种振幅和多种波形； |
| 8）更好地控制在超低或超高应力下的波形； |
| 9）多种波形种类：静态波形、正旋波形、心动波形、三角波形、矩形以及各种te制波形； |
| 10)电脑系统对压力加载周期、大小、频率、持续时间精确智能调控
应用范围： |
| 检测各种组织和细胞在压力作用下的生物化学反应， |
| 例如：胃上皮细胞、肠上皮细胞、软骨组织，椎间盘骨组织，肌腱 |
| 组织，韧带组织，以及从肌肉、肺（肺细胞）、心脏、血管、皮肤、肌腱、韧带、软骨和骨中分离出来的细胞 |
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3、Flex Jr. Tension显微应力加载系统(Flex Jr. Tension) |
| 该系统对显微镜下的细胞提供双轴向应力或单轴向应力加载。 |
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重点应用范围： |
| 心脏、骨骼肌和平滑肌，肺、血管内皮细胞、皮肤、腱、韧带、软骨、骨细胞机械拉伸力加载分析 |
| 4、Tissuetrain三维细胞组织培养系统(flexer-cell Tissue®train System) |
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系统功能亮点： |
| .1)三维细胞应力刺激：对生长在三维状态下的细胞进行静态的或者周期性的应力刺激. |
| .2)三维细胞培养：使用三维组织培养模具和三维细胞培养板可以进行三维细胞培养. |
| .3)三维细胞应力加载： |
| 通过flexer-cell应力加载系统和弧矩形加载平台对生长在三维环境下的细胞进行单轴向或者双轴向的静态或者周期性的应力加载实验.
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| 4)动力模拟实验:可建立te制的各种模拟实验：心率模拟实验，步行模拟实验，跑动模拟实验和其他动力模拟实验. |
| 5)生物人工组织构建：可构建长度达35mm的生物人工组织 |
| 6)观察细胞应力下实时反映：使用标准正立式显微镜实时观察细胞在三维状态下的反应. |
| 7）多种基质蛋白包被的尼龙网锚可以加强细胞与网锚的结合. |
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5、Str-4000细胞流体切应力系统(flexer-cell Fluid Shear Stress Device) |
| 该系统为细胞提供各种形式的流体切应力：稳流式切应力，脉冲式切应力或者往返式切应力，并能通过te显微设备实时观察细胞在机械力刺激下的反应. |
| 美国Glycotech经济、简便平行板流动小室（Parallel-Plate Flow Chamber）
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Vacu-Cell™ Cell Perfusion and Incubation Chambers |
| 二、瑞士InSphero简便、无需支架GravityPLUS三维培养平台 |
| The industry-leading solution for reliable, scaffold-free 3D cell-based assays |
| ●Proven reliability and ease of use with many liquid-handling systems |
| ● SureDrop™ technology assures precise microtissue size – always! |
| ● Includes the GravitytrAP™ plate for long-term culture, assays and imaging |
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三、美国Billups-Rothenberg Inc,MIC-101简便、经济低氧、高氧培养设备 |
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成立于1971年美国比卢普斯罗森堡公司(Billups－rothenberg)的缺氧、常氧、高氧模块化孵化室系统 |
| 已用于世界各地四十年。 |
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该公司生产的缺氧\高氧培养系统可自定义的混合气体和温度组织培养条件.密闭室已被证明安、可靠， |
| 每个单元是一个自包含的孵化器，使您能够创建所需的组织培养的各种环境 (气体浓度和湿度)， |
| 已广泛应用于北美各大实验室和研究机构。该产品获美国，在已发表的论文中引用率在同类产品中是属于高的， |
| 并逐渐被球的实验研人员所使用。 |
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四、英国solentim的Cell Metric和Cell Metric CLD设备
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英国精密仪器制造商Solentim公司已于5月9日推出了新细胞株发展(CLD)成像分析系统Cell Metric。 |
| 据悉，这款具有高分辨率的te细胞成像分析系统为手动检查细胞株单克隆性提供了一种可替代方法， |
| 即对单个细胞克隆进行快速辨识和有效跟踪。此外，自动对焦是Cell Metric性能发挥和确保图像明确且一贯信息的保障。 |
| 另据了解，Solentim公司是一家致力于细胞生物学实验室自动化仪器开发创新的英国公司。 |
| 该团队在生物学识别和解决工作流程方面具有多年的经验。 |
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A fully automated bench-top system for measuring visual, non-invasive cell content of adherent mammalian cells. |
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Images are acquired at single cell resolution for whole microtitre plates using an integrated digital microscope. Cells are imagedin situ, |
| meaning they are not stressed by sample removal (pipetting or enzymic treatement), and since no fluorescent dyes or stains have been added these cells are physiologically relevant for time course studies. |
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Images are processed and presented on the computer with analysis of direct cell number, |
| confluence, colony count or morphology differentiation. Data can be exported and a digital record is created. |
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Key application workflows where Cell Metric™ delivers significant benefits include: |
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Single cell cloning, clonality and cell line development
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| Compound screening, cell proliferation and metabolism |
| Colony counting and clonogenic assays |
| Tissue engineering and stem cells
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A dedicated new high-resolution bench-top imaging system with integrated plate loader, specifically developed to speed up cell line development (CLD).
The unique cell imaging capabilities of the Cell Metric CLD™ enable fast, unequivocal identification and tracking of single-cell derived clones.
Find out more about the key features:
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| Generate cell lines faster |
| Verify mono-clonality |
| Save resources and money |
| Integrated plate loader |
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五、美国动态血脑屏障平台（blood-brain barrier (BBB)）
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Introduction to the Blood-Brain Barrier
TThe blood-brain barrier (BBB) explains why certain substances circulating in the blood are able to get into the brain and others do not. |
| The BBB is formed by brain capillaries that look like simple tubes of endothelial cells. |
| Experimental and clinical evidence suggests that the BBB maintains the chemical environment for neuronal function and protects the brain from harmful substances. |
| The blood-brain barrier is therefore crucial for complex issues such as drug delivery, pathogenesis of chronic neurological diseases, and issues related to bio-defense. |
| We have developed a practical in vitro model that accurately reflects BBB characteristics. |
Benefits of ’s in vitro BBB Model |
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In contrast to other available models of the blood-brain barrier, 's Dynamic In vitro Blood-Brain Barrier (DIV-BBB) respects the anatomical aspects of the in situ endothelial |
| cell-astrocyte interactions and replicates the physiological levels of shear stress to which in situ endothelial cells are exposed. |
| The DIV-BBB allows formation of physiological transendothelial resistance, and formation of gap junctions that can be easily visualized |
| at the electron microscope or determined experimentally with the use of tracers.
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六、美国InQbio细胞生长、培养、成像、监控多功能综合系统
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InQ Cellular Research System |
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The InQ Cell Research System is the first solution that integrates a dynamic software-controlled lab incubator with advanced live cell imaging and real-time data collection. |
| It creates a truein vivoenvironment for studying sensitive human and animal cells for disease research. |
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The system's exclusive capability to tightly control environmental parameters with correlated sample feedback creates a new paradigm in stem cell, neuroscience, |
| and other cell-based research. Scientists will be empowered with novel and more relevant approaches to discovery as they pursue the causes of devastating diseases.
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