mimetas-三维器官芯片OrganoPlate? 2-lane、OrganoPlate? 3-lane、The OrganoPlate? Perfusion Rocker? Mini

型号:9603-400-B、4003-400-B
联系人:程园
联系电话:18611717737
品牌:mimetas

MIMETAS:Organ-on-a-Chip (器官芯片)公司介绍

让复杂的组织研究变得极为简单

 

致力于为最复杂的3D生物学创造最简单的设备和装置。通过对较容易控制的灌注血管,共培养和优化微环境的使用,以至于你不会察觉到自己使用的装置可能是最先进的组织培养平台。通过OrganoPlate®这些愿景都能实现。OrganoPlate® 是3D组织模式的未来。

 

显而易见社会需要更好的药品。而为了达到这一点,需要在充分考虑人类和生理上相关性的基础上,研发更好的疾病模型。作为真正的信息工具,需要在兼容化合物的同时和还具有高通量读出功能。作为organ-on-a-chip生物学真正的先驱者,本公司可开展任何通量的人体组织和疾病模型相关的OrganoPlate®产业。


OrganoPlate®是一种基于微流体技术3D细胞培养板,可在一块板上实现96个单独组织模型的培养。Phaseguides?能够以3D方式精确地重新定义细胞及其培养基,实现细胞间的相互作用以及前所未有品质的成像和定量操作。

OrganoPlate® 2-lane

Product no. 9603-400-B

OrganoPlate 2-lane 400 (Product no. 9603-400-B)

The OrganoPlate 2-lane 400 features 96 independent culture cells, each supporting one in-gel culture and a perfusion channel, that can contain a perfused tubular tissue. The lanes are 400 micron-wide. Four wells of the 384-well-plate user interface connect to each microfluidic network.

The OrganoPlate 2-lane culture cell
Features and applications of the 2-lane and 3-lane OrganoPlate®
Left: Features and applications of the 2-lane and 3-lane OrganoPlate Right: Microfluidic design of a single culture cell

Supported tissue models

The 2-lane OrganoPlate® is a microfluidic 3D culture plate, supporting up to 96 tissue models on a single plate. The OrganoPlate contains Phaseguides?, a proprietary, passive liquid handling technology for membrane-free definition of tissues in microfluidic chambers. The OrganoPlate is based on the industry-standard 384?well plate for full compatibility with liquid handling- and readout equipment.

The OrganoPlate 2-lane 400 supports a range of cell types, including neurons, endothelial tubules, hepatocytes, CaCo2 cells, organoids and kidney epithelial proximal tubules. This plate allows for culture in-gel, tubular culture against the gel and combinations of in-gel and tubular culture.

For more information on tissue model design in the OrganoPlate 2-lane 400, read this.

What is a culture cell in the OrganoPlate®?

A culture cell is a fully autonomous organ-on-a-chip culture unit in the OrganoPlate®. The OrganoPlate 3-lane contains 40 3-lane culture cells, the 2-lane 96 2-lane culture cells, all in a standard 384 well plate format. The culture cells on a single OrganoPlate may be used all at once in an experiment, but can also be used individually.

What is a lane in an OrganoPlate® culture cell?

A lane is an individually addressable part of a culture cell in the OrganoPlate®. The OrganoPlate 3-lane features 3 adjacent lanes per culture cell, 2 of which are perfusable. The OrganoPlate 2-lane features 2 adjacent lanes per culture cells, of which 1 is perfusable. A single lane may contain cells in an ECM gel, a perfused epithelial tubule or just medium flow. By virtue of patented Phaseguide technology, no physical barriers are present between the lanes in the OrganoPlate, allowing co-cultured cells in adjacent lanes to interact and migrate freely.

Materials and equipment

The materials and equipment you need to work with the OrganoPlate® 2-lane 400 are listed below.

  • Cells (cell lines, primary cells, iPSC-derived cells and others)
  • Medium and standard culture materials
  • Extracellular Matrix Gel, for example Collagen I or Matrigel® (Corning®)
  • Mimetas Perfusion Rocker? Mini (optional, please refer to the section below)
  • Pipetting reservoirs 50 ml (e.g. VWR no. 613-1183)
  • Multichannel pipette 5-350 ?L (e.g. Sartorius® no. 730361)
  • Multichannel tips (e.g. Sartorius® 790351)
  • Eppendorf® Repeater® M4 Dispenser (Eppendorf® no. 4982000322)
  • Eppendorf® Combitips advanced® pipette tips Biopur® grade, 0.1 ml     
  • Sartorius® eLINE® Electronic Pipette for volumes between 0.2-10 ?L (Sartorius® no. 730021)
  • Sartorius® 0.1-10 ?L tips (Sartorius® no. 790010)

We will provide you with detailed instructions for use, including materials and equipment.

OrganoPlate® 3-lane

Product no. 4003-400-B

The OrganoPlate 3-lane

The OrganoPlate 3-lane features 40 independent culture cells, each supporting adjacent in-gel culture and 2 perfusion channels, that can contain perfused tubular tissues. The 3-lane models supports apical and basal access to epithelial and endothelial tubules, allowing barrier integrity and transport assays.

 
The new OrganoPlate 3-lane microfluidic design
Features and applications of the 2-lane and 3-lane OrganoPlate
Left: Features and applications of the 2-lane and 3-lane OrganoPlate Right: Microfluidic design of a single culture cell

The lanes are 400 micron wide. Six wells of the 384-well-plate user interface connect to each microfluidic network in a 9x9 well grid, the central well provides viewing access to the microtissue.

Supported tissue models

The OrganoPlate® 3-lane is a microfluidic 3D culture plate, supporting up to 40 tissue models on a single plate. The OrganoPlate contains Phaseguides?, a proprietary, passive liquid handling technology for membrane-free definition of tissues in microfluidic chambers. The OrganoPlate is based on the industry-standard 384?well plate for full compatibility with liquid handling- and readout equipment.

The OrganoPlate 3-lane 400 supports a range of cell types, including neurons, endothelial tubules, hepatocytes, CaCo2 cells, organoids and kidney epithelial proximal tubules. This plate allows for culture in-gel, tubular culture against the gel and combinations of in-gel and tubular culture in 3 lanes, allowing cells to interact through the absence of separating membranes or barriers.

Read this for more information on tissue model design in the OrganoPlate 3-lane 400.

What is a culture cell in the OrganoPlate®?

A culture cell is a fully autonomous organ-on-a-chip culture unit in the OrganoPlate®. The OrganoPlate 3-lane contains 40 3-lane culture cells, a 2-lane 96 2-lane culture cells, all in a standard 384 well plate format. The culture cells on a single OrganoPlate may be used all at once in an experiment, but can also be used individually.

What is a lane in an OrganoPlate® culture cell?

A lane is an individually addressable part of a culture cell in the OrganoPlate®. A 3-lane OrganoPlate features 3 adjacent lanes per culture cell, of which 2 are perfusable. The OrganoPlate 2-lane features 2 adjacent lanes per culture cells, of which 1 is perfusable. A single lane may contain cells in an ECM gel, a perfused epithelial tubule or just medium flow. By virtue of patented Phaseguide technology, no physical barriers are present between the lanes in the OrganoPlate, allowing co-cultured cells in adjacent lanes to interact and migrate freely.

Materials and equipment

The materials and equipment you need to work with the OrganoPlate® 2-lane 400 are listed below.

  • Cells (cell lines, primary cells, iPSC-derived cells and others)
  • Medium and standard culture materials
  • Extracellular Matrix Gel, for example Collagen I or Matrigel® (Corning®)
  • Mimetas Perfusion Rocker? Mini (optional, please refer to the section below)
  • Pipetting reservoirs 50 ml (e.g. VWR no. 613-1183)
  • Multichannel pipette 5-350 ?L (e.g. Sartorius® no. 730361)
  • Multichannel tips (e.g. Sartorius® 790351)
  • Eppendorf® Repeater® M4 Dispenser (Eppendorf® no. 4982000322)
  • Eppendorf® Combitips advanced® pipette tips Biopur® grade, 0.1 ml     
  • Sartorius® eLINE® Electronic Pipette for volumes between 0.2-10 ?L (Sartorius® no. 730021)
  • Sartorius® 0.1-10 ?L tips (Sartorius® no. 790010)

We will provide you with detailed instructions for use, including materials and equipment.

Perfusion Rocker Mini

The MIMETAS Perfusion Rocker Mini

The Perfusion Rocker? Mini provides a continuous medium flow in the OrganoPlate® with minimal settings required. Perfusion Rockers? feature optimized angles, time settings and programs for use with the OrganoPlate®, in contrast to ordinary continuous see-saw rockers.

 Operation

Medium perfusion (flow) in the OrganoPlate® is established by passive leveling of liquids. The perfusion can be maintained indefinitely by placing the OrganoPlate® on a MIMETAS Perfusion Rocker?. This customized rocker platform fits easily in an incubator and enables interval rocking (switching sides every 5-15 minutes), as well as adjustable rocking angles.

Minimal settings are required for pump- and tube-free perfusion in OrganoPlates®. Perfusion Rockers? feature optimized time- and angle settings and programs for use with OrganoPlates®. The Perfusion Rocker comes in two versions: Perfusion Rocker? Mini for 2 stacks of plates and the Perfusion Rocker?Maxi for 4 stacks of plates. Both versions are suitable for use in standard tissue culture incubators, the Perfusion Rocker? Maxi is available on request.