现货VCU动物颅脑损伤仪,细胞损伤控制仪(Cell Injury Controller II),电子脑皮质挫伤撞击仪(eCCI)及液压冲击损伤仪(FPI),脊髓损伤装置,Spinal Cord Injury Device,Weight Drop TBI Model,落重创伤性脑损伤模型,TBI Models

型号:CIC II,eCCI,eCCI
联系人:
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品牌:vcu,cdm

颅脑损伤科研装置大-现货供应

包括:细胞损伤控制仪(Cell Injury Controller II),电子脑皮质挫伤撞击仪(eCCI)及液压冲击损伤仪(FPI),落重创伤性脑损伤模型 (Weight Drop Traumatic Brain Injury Model),脊髓损伤装置(Spinal Cord Injury Device)等。

背景:


    创伤性脑损伤(traumatic brain injuryTBI)是神经外科zui常见的疾病,是导致创伤患者伤残及死亡的主要原因。研究脑损伤后的神经生化、神经病理生理等方面的变化,可为探索行之有效的脑保护治疗提供帮助,将有助于提高颅脑损伤患者的生存率及生存质量。故建立各种便于观察和施加干预因素、控制性佳、可分级、可复制性好并符合人类脑创伤特点的创伤性脑损伤模型,是目前创伤性脑损伤的研究热点。  

VCU动物颅脑损伤仪可以分为细胞损伤控制仪(CIC),电子脑皮质挫伤撞击仪(eCCI)及液压冲击损伤仪(FPI)。这三种产品已经广泛应用于世界范围内的颅脑创伤研究中心,是目前的颅脑创伤模型制作的。同时FPI损伤仪还可应用到眼科损伤模型,CIC细胞损伤仪可以应用到其它种类细胞损伤模型的制作。



产品描述

世联博研北京科技有限公司线销售国际前沿的知名品牌的创伤性脑损伤(traumatic brain injuryTBI)设备,包括vcu大学的液压颅脑损伤仪(FPI),电子颅脑损伤仪(eCCI、细胞颅脑损伤仪(CIC)等系列细胞组织牵张拉伸损伤产品在中国的, 为广大科研用户提供flexercell系列产品,详情致电国免费客服电话:400-650-8506咨询索取资料.



创伤性脑损伤是神经外科常见的疾病,是导致创伤患者伤残及死亡的主要原因。研究脑损伤后的神经生化、神经病理生理等方面的变化,可为探索行之有效的脑保护治疗提供帮助,将有助于提高颅脑损伤患者的生存率及生存质量。故建立各种便于观察和施加干预因素、控制性佳、可分级、可复制性好并符合人类脑创伤特点的创伤性脑损伤模型,是目前创伤性脑损伤的研究热点。 
VCU动物颅脑损伤仪可以分为:

  • 液压颅脑损伤仪(FPI),

  • 电子颅脑损伤仪(eCCI)

  • 细胞颅脑损伤仪(CIC)

这三种产品己广泛应用于世界范围内的颅脑创伤研究中心,是目前的颅脑创伤模型制作的。同时,损伤仪还可应用到眼科损伤模型,细胞损伤仪可以应用到其它种类细胞损伤模型的制作。 
弗吉尼亚州立大学放射学系all定制的设备都是由CDF(定制设计与制造)制作的。2000年的时候,CDF公司(定制设计与制造公司)建立了一个成本核算中心,以便拓展与其他需要定制设备的客户的商业联系。目前CDF公司提供了在机械和电子工程设备定制的面服务。除了网页上面展示的产品外,我们还提供给用户定制服务,希望您有想法告诉我们,我们帮您实现。 

液压颅脑损伤仪(FPI)----现货,当天发货

Fluid Percussion Injury 
液压冲击损伤仪(Fluid Percussion Injury)是由VCU大学所制作设计的,针对神经创伤机制研究。成为球研究神经创伤广泛使用的仪器,基本的组件是采取的Power coating process技术,铝制部分的组件都已经电镀以避免氧化且可以长久使用。液压冲击脑损伤仪可以重复一致的产生液压冲击损伤(FPI)。 
系统点:


  • 可方便的排除气饱。

  • 角度刻度可方便观察撞击角度。

  • 集成压基准力输出,方便校准。可输出j确冲击压力。

  • 配备高精度的压力传感


电子颅脑损伤仪(eCCI)----现货,当天发货

电子颅脑损伤仪(eCCI),electric Cortical Contusion Impactor 
由VCU大学所制作设计的电子大脑皮质挫伤撞击仪(electric Cortical Contusion Impactor),主要针对脑皮质挫伤模型。是神经损伤研究机构受欢迎的损伤模型制作工具。电子大脑皮质挫伤撞击仪(eCCI)的组件有: 坚固的铝架,动物平台,撞击控制器和撞击头。动物平台可以和各种立体定位仪搭配使用。eCCI电子大脑皮质挫伤撞击仪使用高级的线性马达驱动撞击头,并由控制器来控制撞击参数,实现不同程度的损伤。撞击头的组件部分有含感应器,可以确定速率、撞击深度及撞击停留。这些撞击参数完可以重复实现。
与传统Feeney’s自由落体硬膜外撞击方法相比有以下点: 
可j确连续的控制撞击速度,并获得实际撞击深度和停留时间等参数。而非重量差异很大的撞击。由于可j确控制撞击速度和获得实际撞击结果参数,eCCI电子大脑皮质挫伤撞击仪可以j确重复制作挫伤损伤模型。减少动物死亡。使实验过程更加直观,可控。 

细胞颅脑损伤仪(CIC)----现货,当天发货

细胞颅脑损伤仪(CIC),Cell Injury Controller II 
细胞损伤控制仪(Cell Injury Controller II)采取电子式控制,采取电子式控制,适合脑源性细胞培养样品,或其它离体培养细胞的牵张性损伤模型制作。损伤后可进行神经生化、形态学、生理学,药物干预等方面的研究。 
细胞损伤控制仪使用flexercell公司的Tissue Train ? 三维细胞组织应力加载系统。 
细胞损伤控制仪平均把压缩气体送到每个培养室,以造成培养组织牵张性的损伤,损伤的严重程度是依靠控制进出密闭培养室的气体量。养室的峰值压力同时被记录下来,这个数值可以用来j确地表明引起牵张性细胞损伤的气压值。细胞损伤控制仪(CIC II)可以搭配Flex I29.45cm2 柔性基底培养 I (针对VCU早期的细胞损伤控制仪)或BioFlex? 57.75cm2 柔性基底培养板。因为根据所采用的细胞种类、损伤的程度、培养的状况,受损后的细胞或许会因为上述因素死亡或修复,所以VCU的细胞损伤控制仪(CIC II)很适合应用在下列损伤反应研究:细胞受损、修护,死亡,药物介入。


损伤水平

大概膜伸展

Model 94A- Felx I 
膜变形

CIC II-Flex I 
伤等效峰值压力范围

CIC II –BioFlex
等效损伤峰值压力范围

120%

5.5毫米

8.2 - 8.8 PSI

1.8 - 2.0 PSI

135%

6.5毫米

10.0 - 10.5 PSI

2.5 - 3.0 PSI

严重

155%

7.5毫米

11.0 -11.5 PSI

3.5 -4.5 PSI

*Note that the CIC Model 94A Flex I deformation data is the measured distension of a dry reference well. The corresponding peak pressure values are based on the anticipated injury deformation with the addition of 1 ml of fluid media. The presence of 1 ml of fluid increases the deformation approximately 5 percent over the reference well measurement.


落重创伤性脑损伤模型 (Weight Drop Traumatic Brain Injury Model)-----现货,当天发货


小鼠:
长度:25cm
宽度:16厘米
高度:总共25厘米。 底部高度:18cm
包括100克冲击重量
包含蛋泡
包括重量感应管
大鼠:
长度:38cm
宽度:25厘米
高度:总共38厘米。 底部高度:27cm
包括150克冲击重量
包含蛋泡
重量感应管”


  新型的落重型TBI模型使用扫视打击自由移动的啮齿动物的头部,该啮齿动物将加速度,减速度和旋转力传递到大脑。近,越来越多的科学证据表明,儿童时期是由与运动有关的事故,机动车辆伤害和跌落引起的脑震荡所致轻度颅脑外伤的主要危险期。但是,在开发可靠的mTBI动物模型方面一直缺乏进展。尽管用于诱发中度和重度创伤性脑损伤(TBI)的技术的可用性已广泛普及,但这些方法中很少有方法被扩展为在啮齿动物中产生轻度闭合性颅脑损伤。

由于轻度创伤性脑损伤(mTBI)的发生率比中度和重度脑损伤的发生率要高出三倍,因此,越来越需要开发可靠的mTBI模型来协助与生理病理学,神经生物学结果和行为后果和治疗程序。

当对幼鼠进行专门研究时,mTBI体重减轻模型会产生临床相关的行为结果,这些结果可描述脑震荡后的症状。这种方法的简单和基本性质为研究人员提供了可靠的mTBI模型,该模型可广泛用于行为,分子和遗传研究。



MTBI装置通常包括两个主要部分:U形的有机玻璃盒和收集海绵。

典型的mTBI重量减轻设备包括一个U形有机玻璃支架(38 x 27 x 27cm3),一个塑料导管,黄铜砝码,夹具支架,钓线和一个收集海绵(38 x 18 x 25 cm3)。 将轻度麻醉的啮齿动物仰卧放置在刻痕的锡箔上,其头直接位于重量下降的路径中。

该模型包括局部脑损伤模型和弥漫性脑损伤模型两种。


Protocol

All animals must be housed with freely accessible food and water along with properly maintained optimal room temperature. 30 days Post-natal, juvenile rodents can be used for taking part in the experiment.

The overall goal of the weight-drop mTBI model is to generate mild traumatic brain injury in juvenile rodents. This is accomplished by first preparing the injury induction platform. Attach a metal loop to the top end of the desired weight allowing the fishing line to be fastened to the weight. A tin foil is sd with a razor sharp blade making certain that the sd tin foil supports the body weight of the rodent. The foil is then placed on the U-shaped stage made of Plexiglas and securely taped to it. The U-shaped plastic stage is placed in the accurate position under the guide tube made of clear plastic. The plastic guide tube is held in place with a clamp stand and positioned above the sd tin foil. The fishing line is then attached through the metal loop to the weight ensuring that the bottom of the weight hangs freely over the sd tin foil. The fishing line is then connected to the clamp stand, and the weight is drawn up through the plastic guide tube with the fishing line and secured in its position with an Allen key pin.

Next, a rodent is taken and lightly anesthetized until it is non-responsive to a paw or tail pinch. The anesthetized rodent is placed face down on the sd tin foil. Once in place, the Allen key pin is pulled allowing the weight to drop and generate a glancing blow to the head. The animal swiftly undergoes a 180° rotation and lands on the collection sponge. The animal is then placed in a supine position and the time it takes to right itself is recorded.

Like any technical method, certain stages of the protocol are predominantly important to generate reliable outcomes. Primarily, the tin foil should be sd properly to produce the desired effect. If the tin foil is not effectively sd, the force exerted by the weight for the duration of the glancing blow will not be sufficient to thrust the juvenile rodent through the tin foil onto the collection sponge. Hence, the desired rotational acceleration and deceleration will not occur. Secondly, during the induction of the mTBI injury, the level of anesthetic that each rodent is exposed to should remain consistent. An important benefit of this technique over countless other TBI methods is the low level and duration of anesthesiology. However, the juvenile rodent must be non-responsive to a toe or tail pinch to make certain that they do not wake-up on the stage before the injury is produced.

Applications

Reliable animal models for inducing mild traumatic brain injury can make use of the modified weight drop mTBI device to induce an injury that strongly embodies the pathophysiology and symptoms related to concussions and repetitive mTBI in the adolescent populations. The implications of this technique extend towards therapy of pediatric concussion and mild traumatic brain injury because the model induces clinically relevant symptomology in a heterogeneous fashion.

The present definition of mTBI states that the injury must be the outcome of acceleration and deceleration forces affiliated with blunt trauma. Therefore, the modified weight drop model described above is the ideal method to use in experiments aiming to study mTBI and concussion-like injuries as it makes use of a glancing impact to transmit quick rotational acceleration and deceleration forces to the brain of the unrestrained animal. These forces imitate the biomechanical forces specifically linked to sports-related injuries and motor vehicle accidents (Mychasiuk et al., 2014).

Additionally, this model can be easily modified to observe repetitive mTBI, an incidence that is rising as a severe medical and socioeconomic issue. Evidence indicates that rodents may possibly be exposed to up to 10 distinctive mTBIs with minimum mortality rates. Furthermore, the technique is inexpensive and can be executed quickly, allowing for a highly thorough examination of numerous therapeutic compounds and treatment controls.

Strengths And Limitations

The main advantage of the weight drop mTBI model over others is that the animals are only briefly anesthetized. While the majority of the present TBI techniques impose such intense injuries, it is frequently hard to induce another injury and almost impossible to study repetitive TBI without widespread damage to the entire cortex. However, in the weight drop mTBI technique, a closed head injury is inflicted with no overt damage to the brain which allows the researcher to repeat the injury in the same animal several times.

Another advantage of the weight drop mTBI model is that there’s a rotational component to the injury that generates a more clinically relevant concussive-like symptomology. On the basis of the biomechanical pathophysiology of the induced injury and the behavioral outcomes studied, the modified weight-drop mTBI technique has emerged as a dependable model for the examination of pediatric TBI and concussion. Although preliminary studies of this new model have detected some fundamental molecular and structural transformations, further studies will be required to determine how the brain acts in response to a TBI with this injury etiology.

Summary

  • The weight-drop TBI apparatus is usually comprised of a U-shaped Plexiglas box and a collection sponge and makes use of a plastic guide tube, brass weights, clamp stand, and fishing line.
  • The weight drop mTBI model is a technique that generates a glancing blow to the head of a freely moving rodent.
  • The blunt force, in turn, transmits acceleration, deceleration, and rotational forces on to the brain that generates more clinically relevant symptoms.
  • The closed head injury allows the researcher to study the outcomes of repetitive mTBI in the same animal.

References

Mychasiuk, R., Farran, A., Angoa-Perez, M., Briggs, D., Kuhn, D., Esser, M. J. A Novel Model of Mild Traumatic Brain Injury for Juvenile Rats. J. Vis. Exp. (94), e51820, doi:10.3791/51820 (2014).






脊髓损伤装置(Spinal Cord Injury Device----现货,当天发货


能够对实验动物对象脊髓腹侧进行撞击实验,造成腹侧脊髓相应程度的损伤脊髓损伤装置


SCI设备是使用插入铁氟龙底座中的钢制蓄能器构造的。 缓冲器通过水平销钉连接到空心管的末端,以引导砝码并防止其在撞击时弹跳。 配重由te氟隆涂层不锈钢制成,并由可移动的销支撑,该销也用于释放配重。 棒状磁铁用于在受伤后恢复体重。

Spinal cord injury usually results from trauma and can also be the result of diseases or degeneration. Depending on the severity of the injury, SCI can result in severe sensory/motor dysfunction, secondary injuries that could result in tissue damage and cell death, glial scar formation, and impaired regeneration. Apart from the injuries, sufferers of SCI also tend to experience chronic pain that impacts their everyday life.

Since there exists no curative treatment for SCI, establishing an ideal animal model to mirror human injuries is crucial for the identification of the injury mechanism and its effects on the capabilities of its sufferer. The novel SCI Device is modeled after the Weight Drop model, considered as a standard experimental spinal cord contusion injury model designed by Alfred Reginald Allen in 1911 (Koozekanani et al., 1976). Allen’s spinal cord contusion technique was iterated over the years, but Ahdeah Pajoohesh-Ganji and colleagues’ version is a novel yet efficient method for spinal cord contusion.

The SCI device is constructed using a steel impounder inserted into a Teflon base. The impounder is attached to the end of the hollow tube by a horizontal pin to guide the weight and to prevent it from bouncing on impact. The weight is made of Teflon coated stainless steel and is supported by a removable pin which is also used to release the weight. Rod magnet is used for retrieval of the weight after the injury.

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Apparatus And Equipment

A hollow Teflon tube of length 25 cm and diameter 6 mm is used to house the impounder and the weight. The steel impounder is attached to a horizontal pin that helps guide the weight and prevent the bouncing of the weight on impact. The impounder is 3 cm in height and 5 mm in diameter and has a 1 cm needle of diameter 1.2 mm on its end. The height of the drop can be adjusted to 10 mm or 20 mm above the impounder for mild-moderate or moderate-severe injury, respectively. This is done with the help of a removable pin that supports the Teflon coated stainless steel weight and also functions as the release mechanism for the weight. The retrieval of the weight is done using a rod magnet lowered into the hollow tube.

Protocol

The subject is anesthetized using Isoflurane, and a laminectomy is performed at the desired site of injury. The stabilization of the spinal cord is done using transverse clamps. The contusive injury is performed using a weight of 1.85 g released from 10 mm or 20 mm for mild-moderate or moderate-severe injury respectively. The impounder must be placed perpendicularly in the center of the spinal cord at the time of impact to ensure symmetrical injury.

The SCI device has been used in studies involving mouse models to study the hind-limb functional performance after contusion SCI at T9 (Pajoohesh-Ganji et al., 2010).

Strengths And Limitations

Strengths 

The rats have been predominantly used in the investigatory studies and experiments of spinal cord injuries. But with the availability of transgenic animals, there has been an increase in the usage of mouse models. The SCI device can be successfully used for inducing spinal cord injuries in mice enabling investigation of the correlation between behavioral tests and the injury severity or tissue damage. The research strongly backs the inverse correlation between the severity of injury and white matter. The white matter decreases with the increased severity of the injury (McEwen and Springer, 2006). The preciseness of the modified SCI device to manipulate the extent of the injury is critical in achieving more spared peripheral white matter.

Limitations

The position of the impounder plays a critical role in inducing symmetrical injuries. Therefore, the impounder must be placed perpendicularly in the center of the spinal cord at the time of impact to ensure symmetrical injury

Summary

  • The device uses a removable pin to both support the weight at the ideal height and to function as a release mechanism
  • The impounder is held in place using a horizontal pin which guides the weight and also prevents it from bouncing on impact
  • Retrieval of the weight is done using a rod magnet
  • The impounder must be placed perpendicular to the spinal cord to induce symmetrical injuries

References

Koozekanani SH, Vise WM, Hashemi RM, McGhee RB. (1976). Possible mechanisms for observed pathophysiological variability in experimental spinal cord injury by the method of Allen. J Neurosurg.  Apr; 44(4):429-34.

Ahdeah Pajoohesh-Ganji, Kimberly R. Byrnes, Gita Fatemi, Alan I. Faden. (2010). A combined scoring method to assess behavioral recovery after mouse spinal cord injury. Neurosci Res; 67(2): 117–125.

Akhtar AZ, Pippin JJ, Sandusky CB. (2008). Animal models in spinal cord injury: a review.Rev Neurosci; 19:47–60.

McEwen, M.L., Springer, J.E. (2006). Quantification of locomotor recovery following

spinal cord contusion in adult rats. J. Neurotrauma 23, 1632–1653.