AIM 的解决方案：VasQ 试剂盒用于生成可灌注 3D MVN 的一体化试剂盒主要特点- 现实。模拟人体组织的自组装脉管系统。
- 快。在 1 周内实现可灌注 3D MVN。
- 精简。无需泵或板摇杆。
- 自动化。与自动成像和液体处理兼容。
- 重现。标准化的细胞来源可确保一致性并克服批次间差异。
产品详情- 一体化解决方案。VasQ 试剂盒简化了 MVN 的生成，使研究人员能够创建、测试和分析人类相关的血管生物学。
- 标准化细胞来源。该试剂盒包括 hTERT 永生化人脐静脉内皮细胞 （HUVEC） 和肺成纤维细胞 （LF），它们保留了原代细胞表型，同时确保长期可用性以获得一致的结果。在此处了解有关 hTERT 永生化细胞来源优势的更多信息，并在此处查看比较研究。
- 更高水平的可重复性。我们的团队确保每件产品的质量，为研究人员提供一致的血管形成。
- 自组装的脉管系统。在纤维蛋白凝胶中与肺成纤维细胞共培养的内皮细胞自组装成 3D MVN，模拟体内血管发生过程并建立真实的细胞-细胞和细胞-ECM 相互作用。
Selected Publications Using AIM’s TechnologyVasculogenesis- A Robust Method for Perfusable Microvascular Network Formation In Vitro. Wan, Z., Zhong, A. X., Zhang, S., Pavlou, G., Coughlin, M. F., Shelton, S. E., … & Kamm, R. D. (2022). Small Methods, 6(6), 2200143.
- Physiologic flow-conditioning limits vascular dysfunction in engineered human capillaries. Haase, K., Piatti, F., Marcano, M., Shin, Y., Visone, R., Redaelli, A., … & Kamm, R. D. (2022). Biomaterials, 280, 121248.
- A robust vasculogenic microfluidic model using human immortalized endothelial cells and Thy1 positive fibroblasts. Wan, Z., Zhang, S., Zhong, A. X., Shelton, S. E., Campisi, M., Sundararaman, S. K., … & Kamm, R. D. (2021). Biomaterials, 276, 121032.
- Tuning the local availability of VEGF within glycosaminoglycan‐based hydrogels to modulate vascular endothelial cell morphogenesis. Limasale, Y. D. P., Atallah, P., Werner, C., Freudenberg, U., & Zimmermann, R. (2020). Advanced Functional Materials, 30(44), 2000068.
Oncology Applications- Design and Discovery of New Collagen V-Derived FGF2-Blocking Natural Peptides Inhibiting Lung Squamous Cell Carcinoma In Vitro and In Vivo. Kuang, K., Chen, X., Wang, M., Han, W., Qiu, X., Jin, T., … & Jia, T. (2024). Journal of Medicinal Chemistry, 67(15), 12660-12675.
- The effects of luminal and trans-endothelial fluid flows on the extravasation and tissue invasion of tumor cells in a 3D in vitro microvascular platform. Hajal, C., Ibrahim, L., Serrano, J. C., Offeddu, G. S., & Kamm, R. D. (2021). Biomaterials, 265, 120470.
- Tumor-Derived cGAMP Regulates Activation of the Vasculature. Campisi, M., Sundararaman, S. K., Shelton, S. E., Knelson, E. H., Mahadevan, N. R., Yoshida, R., … & Barbie, D. A. (2020). Frontiers in Immunology, 11, 2090.
- On-chip human microvasculature assay for visualization and quantification of tumor cell extravasation dynamics. Chen, M. B., Whisler, J. A., Fr?se, J., Yu, C., Shin, Y., & Kamm, R. D. (2017). Nature Protocols, 12(5), 865-880.
For more publications using our technology to study vascular biology, visit our website publications page.
使用AngioPrime检测就绪解决方案简化并标准化您的药物筛选和开发过程。主要特点- 有效。在 AIM 的 idenTx 40 板中预接种的内皮细胞，到达后即可发芽。
- 快。仅 4 天内即可完成血管生成。
- 精简。无需泵或板摇杆。
- 自动化。与自动成像和液体处理兼容。
- 重现。 使用标准化细胞来源时，无批次间差异。
AIM在idenTx 40中填充胶原蛋白和种子内皮细胞。 AIM进行质量控制并在球范围内运送化验就绪的产品。 它是如何工作的？- I 型胶原蛋白被加载到 idenTx 40 的中央凝胶通道中，形成 3D 水凝胶以支持血管生成发芽。
- 标准化人脐静脉内皮细胞 （HUVEC） 接种在顶部培养基通道中，准备发芽成胶原蛋白。
- 两个媒介类渠道都添加了专门的配送媒介，以保持配送过程中的稳定性并改善送达后的恢复率。
- 细胞恢复在使用前进行。
- 3 天后，可以观察到血管生成发芽，并且可以应用不同的化合物或测试条件来评估血管生成活性。
产品详情Selected Publications Using AIM’s TechnologyAngiogenesis- A streamlined method to generate endothelial cells from human pluripotent stem cells via transient doxycycline-inducible ETV2 activation. Luo, A. C., Wang, J., Wang, K., Zhu, Y., Gong, L., Lee, U., … & Melero-Martin, J. M. (2024). Angiogenesis, 27(4), 779-795.
- The 3D organ-on-a-chip model unveils a dual role of GDF-15 in vascular growth. Sarad, K., Dulak, J., & Ja?wa-Kusior, A. (2024). Biochemical and Biophysical Research Communications, 733, 150441.
- 3D Microvascularized Tissue Models by Laser-Based Cavitation Molding of Collagen. Enrico, A., Voulgaris, D., ?stmans, R., Sundaravadivel, N., Moutaux, L., Cordier, A., … & Stemme, G. (2022). Advanced Materials, 34(11), 2109823.
- Podoplanin is Responsible for the Distinct Blood and Lymphatic Capillaries. Jeong, D. P., Hall, E., Neu, E., & Hanjaya-Putra, D. (2022). Cellular and molecular bioengineering, 15(5), 467-478.
- Angiogenic Sprouting Dynamics Mediated by Endothelial-Fibroblast Interactions in Microfluidic Systems. Walji, N., Kheiri, S., & Young, E. W. (2021). Advanced Biology, 5(11), 2101080.
Anti-Angiogenesis- Exosomal miR-184 in the aqueous humor of patients with central serous chorioretinopathy: a potential diagnostic and prognostic biomarker. Yang, J. M., Kim, S. J., Park, S., Son, W., Kim, A., & Lee, J. (2023). Journal of Nanobiotechnology, 21(1), 242.
- Antiangiogenic Nanomicelles for the Topical Delivery of Aflibercept to Treat Retinal Neovascular Disease. Zhao, X., Seah, I., Xue, K., Wong, W., Tan, Q. S. W., Ma, X., … & Loh, X. J. (2022). Advanced Materials, 34(25), 2108360.
- Isolinderalactone suppresses human glioblastoma growth and angiogenic activity in 3D microfluidic chip and in vivo mouse models. Park, J. H., Kim, M. J., Kim, W. J., Kwon, K. D., Ha, K. T., Choi, B. T., … & Shin, H. K. (2020). Cancer letters, 478, 71-81.
- Phthalimide Derivative Shows Anti-angiogenic Activity in a 3D Microfluidic Model and No Teratogenicity in Zebrafish Embryos. Mercurio, A., Sharples, L., Corbo, F., Franchini, C., Vacca, A., Catalano, A., … & Adriani, G. (2019). Frontiers in pharmacology, 10, 349.
For more publications using our technology to study vascular biology, visit our website publications page.
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