BHQ® Probe Master Mix is a versatile 2X PCR master mix specially designed to deliver optimal SNP genotyping results for high-throughput, ultra low volume, endpoint probe-based SNP genotyping. BHQ Probe Master Mix frees you to use an extraction method of your choice across a range of probe, sample, and template types. For high-precision genotyping, pair BHQ Probe Master Mix with BHQ or BHQplus® probes for a high-fidelity, low background SNP genotyping assay that yields clear results minus the challenges associated with ambiguous data interpretation.
Browse All BHQ probes master mix
If ordering ValuMix Assay for SNP Genotyping, which BHQ Probe Master Mix kit volume should I order?
ValuMix Assays are optimized to deliver the optimal probe/primer ratio for a final primer/probe concentration of 200 µM in a single tube. All ValuMix Assays contain 2 x BHQplus probes, one with a FAM labeled 5' end and another with a dye of your choice TET, CAL Fluor® Gold 540, CAL Fluor Orange 560.
All ValuMix probes are reverse-phase HPLC (RP HPLC) purified and combined with salt-free primers.
| ValuMix for SNP genotyping (probe concentration) | ValuMix Catalog Number(s) | BHQ Probe Master Mix minimum kit volume | BHQ Probe Master Mix Catalog Number(s) |
|---|---|---|---|
| 2 nmol | FTBP-2 (FAM/TET) FCBP-2 (FAM/CAL Fluor Orange 560)) FGBP-2 (FAM/CAL Fluor Gold 540) |
5 mL | KBS-1040-003 No ROX KBS-1040-013 Low ROX KBS-1040-023 Standard ROX |
| 5 nmol | FTBP-5 (FAM/TET) FCBP-5 (FAM/CAL Fluor Orange 560) FGBP-5 (FAM/CAL Fluor Gold 540) |
25 mL | KBS-1040-005 No ROX KBS-1040-015 Low ROX KBS-1040-025 Standard ROX |
| 12 nmol | FTBP-12 (FAM/TET) FCBP-12 (CAL Fluor Orange 560) FGBP-12 (CAL Fluor Gold 540) |
200 mL | KBS-1040-006 No ROX KBD-1040-016 Low ROX KBS-1040-026 Standard ROX |
Ready to purchase BHQ Probes Master Mix? Choose from the options below.
| Catalog Number | Description | Size | Order |
|---|---|---|---|
| KBS-1040-001 | 2X BHQ Probe Master Mix, No ROX | 1.25 mL | Order Now |
| KBS-1040-002 | 2X BHQ Probe Master Mix, No ROX | 2.5 mL | Inquire |
| KBS-1040-003 | 2X BHQ Probe Master Mix, No ROX | 5 mL | Order Now |
| KBS-1040-004 | 2X BHQ Probe Master Mix, No ROX | 10 mL | Inquire |
| KBS-1040-005 | 2X BHQ Probe Master Mix, No ROX | 25 mL | Inquire |
| KBS-1040-006 | 2X BHQ Probe Master Mix, No ROX | 200 mL | Inquire |
| KBS-1040-011 | 2X BHQ Probe Master Mix, Low ROX | 1.25 mL | Order Now |
| KBS-1040-012 | 2X BHQ Probe Master Mix, Low ROX | 2.5 mL | Inquire |
| KBS-1040-013 | 2X BHQ Probe Master Mix, Low ROX | 5 mL | Order Now |
| KBS-1040-014 | 2X BHQ Probe Master Mix, Low ROX | 10 mL | Inquire |
| KBS-1040-015 | 2X BHQ Probe Master Mix, Low ROX | 25 mL | Inquire |
| KBS-1040-016 | 2X BHQ Probe Master Mix, Low ROX | 200 mL | Inquire |
| KBS-1040-021 | 2X BHQ Probe Master Mix, Standard ROX | 1.25 mL | Order Now |
| KBS-1040-022 | 2X BHQ Probe Master Mix, Standard ROX | 2.5 mL | Inquire |
| KBS-1040-023 | 2X BHQ Probe Master Mix, Standard ROX | 5 mL | Order Now |
| KBS-1040-024 | 2X BHQ Probe Master Mix, Standard ROX | 10 mL | Inquire |
| KBS-1040-025 | 2X BHQ Probe Master Mix, Standard ROX | 25 mL | Order Now |
| KBS-1040-026 | 2X BHQ Probe Mastermix, Standard ROX | 200 mL | Inquire |
BHQ Probe Master Mix
Competing master mix
Figure 1: A direct comparison of BHQ Probe Master Mix to that of a leading market competitor on crudely extracted and challenging sunflower seed samples known to contain inhibitors. BHQ Probe Master Mix shows tight and easily resolvable clusters with limited no template control (NTC) movement even in the presence of sample inhibitors present in higher concentrations due to the very low total reaction volume of 0.8 渭L. Competing mix shows incomplete amplification due to inhibitors resulting in samples where genotype cannot be assigned (pink circles). Experiments adhered to respective mix protocols on hot shot extracted sunflower seed samples using BHQplus Probes on a Nexar庐 Integrated Liquid Handling and Assay Processing System.
Storage and Handling
BHQ Probe Master Mix is stable for up to one week at 4 °C, 1-year at -20 °C.
Instrument Compatibility
Nexar Inline Liquid Handling and Assay Processing System
IntelliQube®
SNPline™ workflow of modular laboratory instrumentation, which includes the Hydrocycler
Other Platforms
Any appropriate qPCR instrument of FRET- capable plate reader
Related Links
How many 10 µL reactions can I get with each kit?
| Kit | 10 µL Reactions |
|---|---|
| 1.25 mL | 250 |
| 2.5 mL | 500 |
| 5 mL | 1000 |
| 10 mL | 2000 |
| 25 mL | 5000 |
| 200 mL | 40,000 |