产品编号 9603-400-B
OrganoPlate 2泳道400具有96个立的培养细胞，每个细胞支持一个凝胶内培养和灌注通道，可以包含灌注的管状组织。车道宽400微米。384孔板用户界面的四个孔连接到每个微流体网络。
mimetas-三维器官芯片OrganoPlate? 2-lane、OrganoPlate? 3-lane、The OrganoPlate? Perfusion Rocker? Mini
型号:9603-400-B、4003-400-B
联系人:程园
联系电话:18611717737
品牌:mimetas
MIMETAS：Organ-on-a-Chip （器官芯片）公司介绍
让复杂的组织研究变得j为简单
于为复杂的3D生物学创造简单的设备和装置。通过对较容易控制的灌注血管，共培养和化微环境的使用，以至于你不会察觉到自己使用的装置可能是的组织培养平台。通过OrganoPlate®这些愿景都能实现。OrganoPlate® 是3D组织模式的未来。
显而易见社会需要更好的药品。而为了达到这一点，需要在充分考虑人类和生理上相关性的基础上，研发更好的疾病模型。作为真正的信息工具，需要在兼容化合物的同时和还具有高通量读出功能。作为organ-on-a-chip生物学真正的先驱者，本公司可开展任何通量的人体组织和疾病模型相关的OrganoPlate®产业。
OrganoPlate®是一种基于微流体技术3D细胞培养板，可在一块板上实现96个单组织模型的培养。Phaseguides?能够以3D方式j确地重新定义细胞及其培养基，实现细胞间的相互作用以及前所未有品质的成像和定量操作。
OrganoPlate® 2-lane
图1：单个培养细胞的微流体设计 图2：2车道和3车道OrganoPlate的特点和应用Supported tissue models
The 2-lane OrganoPlate® is a microfluidic 3D culture plate, supporting up to 96 tissue models on a single plate.
The OrganoPlate contains Phaseguides?, a proprietary, passive liquid handling technology for membrane-free definition of tissues in microfluidic chambers.
The OrganoPlate is based on the industry-standard 384?well plate for full compatibility with liquid handling- and readout equipment.
The OrganoPlate 2-lane 400 supports a range of cell types, including neurons, endothelial tubules, hepatocytes, CaCo2 cells, organoids and kidney epithelial proximal tubules.
This plate allows for culture in-gel, tubular culture against the gel and combinations of in-gel and tubular culture.
What is a culture cell in the OrganoPlate®?
A culture cell is a fully autonomous organ-on-a-chip culture unit in the OrganoPlate®.
The OrganoPlate 3-lane contains 40 3-lane culture cells, the 2-lane 96 2-lane culture cells, all in a standard 384 well plate format.
The culture cells on a single OrganoPlate may be used all at once in an experiment, but can also be used individually.
What is a lane in an OrganoPlate® culture cell?
A lane is an individually addressable part of a culture cell in the OrganoPlate®. The OrganoPlate 3-lane features 3 adjacent lanes per culture cell, 2 of which are perfusable.
The OrganoPlate 2-lane features 2 adjacent lanes per culture cells, of which 1 is perfusable. A single lane may contain cells in an ECM gel, a perfused epithelial tubule or just medium flow.
By virtue of patented Phaseguide technology, no physical barriers are present between the lanes in the OrganoPlate, allowing co-cultured cells in adjacent lanes to interact and migrate freely.
Materials and equipment
The materials and equipment you need to work with the OrganoPlate® 2-lane 400 are listed below.
- Cells (cell lines, primary cells, iPSC-derived cells and others)
- Medium and standard culture materials
- Extracellular Matrix Gel, for example Collagen I or Matrigel® (Corning®)
- Mimetas Perfusion Rocker? Mini (optional, please refer to the section below)
- Pipetting reservoirs 50 ml (e.g. VWR no. 613-1183)
- Multichannel pipette 5-350 ?L (e.g. Sartorius® no. 730361)
- Multichannel tips (e.g. Sartorius® 790351)
- Eppendorf® Repeater® M4 Dispenser (Eppendorf® no. 4982000322)
- Eppendorf® Combitips advanced® pipette tips Biopur® grade, 0.1 ml
- Sartorius® eLINE® Electronic Pipette for volumes between 0.2-10 ?L (Sartorius® no. 730021)
- Sartorius® 0.1-10 ?L tips (Sartorius® no. 790010)
We will provide you with detailed instructions for use, including materials and equipment.
OrganoPlate ® 3车道
产品编号 4003-400-B
OrganoPlate 3通道具有40个立的培养细胞，每个细胞支持相邻的凝胶内培养和2个灌注通道，可以包含灌注的管状组织。3通道模型支持上皮和内皮小管的顶端和基底通路，允许屏障完整性和运输测定。
图1：单个培养细胞的微流体设计 图2：2车道和3车道OrganoPlate的特点和应用
车道宽400微米。384孔板用户界面的六个孔连接到9x9孔网格中的每个微流体网络，中心孔提供对微组织的观察访问。
Supported tissue models
The OrganoPlate® 3-lane is a microfluidic 3D culture plate, supporting up to 40 tissue models on a single plate. The OrganoPlate contains Phaseguides?, a proprietary, passive liquid handling technology for membrane-free definition of tissues in microfluidic chambers. The OrganoPlate is based on the industry-standard 384?well plate for full compatibility with liquid handling- and readout equipment.
The OrganoPlate 3-lane 400 supports a range of cell types, including neurons, endothelial tubules, hepatocytes, CaCo2 cells, organoids and kidney epithelial proximal tubules. This plate allows for culture in-gel, tubular culture against the gel and combinations of in-gel and tubular culture in 3 lanes, allowing cells to interact through the absence of separating membranes or barriers.
Read this for more information on tissue model design in the OrganoPlate 3-lane 400.
What is a culture cell in the OrganoPlate®?
A culture cell is a fully autonomous organ-on-a-chip culture unit in the OrganoPlate®. The OrganoPlate 3-lane contains 40 3-lane culture cells, a 2-lane 96 2-lane culture cells, all in a standard 384 well plate format. The culture cells on a single OrganoPlate may be used all at once in an experiment, but can also be used individually.
What is a lane in an OrganoPlate® culture cell?
A lane is an individually addressable part of a culture cell in the OrganoPlate®. A 3-lane OrganoPlate features 3 adjacent lanes per culture cell, of which 2 are perfusable. The OrganoPlate 2-lane features 2 adjacent lanes per culture cells, of which 1 is perfusable. A single lane may contain cells in an ECM gel, a perfused epithelial tubule or just medium flow. By virtue of patented Phaseguide technology, no physical barriers are present between the lanes in the OrganoPlate, allowing co-cultured cells in adjacent lanes to interact and migrate freely.
Materials and equipment
The materials and equipment you need to work with the OrganoPlate® 2-lane 400 are listed below.
- Cells (cell lines, primary cells, iPSC-derived cells and others)
- Medium and standard culture materials
- Extracellular Matrix Gel, for example Collagen I or Matrigel® (Corning®)
- Mimetas Perfusion Rocker? Mini (optional, please refer to the section below)
- Pipetting reservoirs 50 ml (e.g. VWR no. 613-1183)
- Multichannel pipette 5-350 ?L (e.g. Sartorius® no. 730361)
- Multichannel tips (e.g. Sartorius® 790351)
- Eppendorf® Repeater® M4 Dispenser (Eppendorf® no. 4982000322)
- Eppendorf® Combitips advanced® pipette tips Biopur® grade, 0.1 ml
- Sartorius® eLINE® Electronic Pipette for volumes between 0.2-10 ?L (Sartorius® no. 730021)
- Sartorius® 0.1-10 ?L tips (Sartorius® no. 790010)
We will provide you with detailed instructions for use, including materials and equipment.
Perfusion Rocker Mini
The Perfusion Rocker? Mini provides a continuous medium flow in the OrganoPlate® with minimal settings required. Perfusion Rockers? feature optimized angles, time settings and programs for use with the OrganoPlate®, in contrast to ordinary continuous see-saw rockers.
Operation
Medium perfusion (flow) in the OrganoPlate® is established by passive leveling of liquids. The perfusion can be maintained indefinitely by placing the OrganoPlate® on a MIMETAS Perfusion Rocker?. This customized rocker platform fits easily in an incubator and enables interval rocking (switching sides every 5-15 minutes), as well as adjustable rocking angles.
Minimal settings are required for pump- and tube-free perfusion in OrganoPlates®. Perfusion Rockers? feature optimized time- and angle settings and programs for use with OrganoPlates®. The Perfusion Rocker comes in two versions: Perfusion Rocker? Mini for 2 stacks of plates and the Perfusion Rocker?Maxi for 4 stacks of plates. Both versions are suitable for use in standard tissue culture incubators, the Perfusion Rocker? Maxi is available on request.
