AFS单分子原子力谱仪,高通量单分子原子力显微镜,Single Molecule Atomic Force Spectrometer, 单分子力谱仪,Atomic-Force-Spectroscope,蛋白质动力学定量测量系统

型号:Single Molecule Atomic Force Spectrometer
联系人:李先生
联系电话:18618101725
品牌:德国

德国AFS单分子原子力谱仪 (Single Molecule Atomic Force Spectrometer)

新型,高线性原子力光谱仪,用于蛋白质动力学的定量测量
该装置可对单个分子进行高通量分析,阐明诸如解折叠速率和保持其构象的键强度等特征。根据要确定的分子的性质,此设备可以与多种协议一起使用:力扩展,力钳,力斜坡和各种重新折叠协议。
  • 力钳和力-延伸
  • 亚纳米分辨率
  • 亚毫秒级时间分辨率
  • 蛋白质折叠和展开
  • 键裂解和形成
  • 自动操作
  • 强大的分析软件
  • 简单的用户界面
  • 高通量

原子力光谱仪(AFS)是用于在校准的机械负载下单个蛋白研究的仪器.
AFS用于了解机械力在整个生物光谱,影响蛋白质的动力学和化学性质。
AFS允许拾取和机械操纵单个重组蛋白。 该系统自动,可以连续运行数天,wu需人看管。
操作软件中包含的例程明确识别所研究蛋白质的机械指纹,并因此能够
自动识别和存储数据。在为期一天的实验结束后,可以收集多个一百种单蛋白痕迹。

用于AFS测量的蛋白质附着的标准方法是使用硫醇化学方法,该方法是通过在覆盖有金的玻璃盖玻片上将在末端含有半胱氨酸锚定分子的重组蛋白分层来获得的。 zui有效的方法是结合使用HaloTag(Promega)技术和硫醇化学方法,该方法可以很容易地将共价连接的蛋白质传递给两者。 镀金的悬臂和Halo-配体的玻璃盖玻片。 共价连接的蛋白质可以长时间进行机械操作。
选的蛋白质样品被安排为串联模块蛋白质。 当此类多蛋白通过AFS铺展时,其作用力是te的机械指纹,可将它们与困扰单分子研究的更常见的非te异性事件明确区分开。


This unique Atomic-Force-Spectroscope (AFS) , based on the Atomic Force Microscope Technology (AFM) was specially designed for studying single proteins placed under a calibrated mechanical load.
The upside down design, where the cantilever, laser and sensor is fixed while the substrate with attached protein is moved by a ultra-precise linear Piezo, enables us to get a feedback response time better than 1ms and a position accuracy better than 1nm.
The software is based on IGOR and enables the operator to run automatic experiments over the day without putting hands on.
The AFS can be operated in two modes:
- force extension (constant velocity)
- force clamp (constant force)
In force clamp mode the operator can design his own force protocol to measure protein folding, unfolding and protein chemical reactions.
The software includes routines that unambiguously identify the mechanical fingerprints of the protein being studied, and thus is able to recognize and store data automatically.
A tuneable Proportional-Integral-Differential system (PID) enables the operator to adjust the parameter for the Piezo, cantilever and probe to get the best feedback time for each individual experiment.
The AFS comes as a complete workstation, incl. vibration damping table, PC Monitor and software.
For further information please visit the web page of Professor Julio Fernandez, in which collaboration this system was designed.



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