有限压缩多孔弹性模型装置系统,Poroelastic Model in Confined Compression,双相材料侧限压缩仪,CONFINED COMPRESSION tester,无膨胀变形侧限压缩测试系统,受限压缩多孔弹性模型分析系统,关节软骨侧限压缩力测试系统,软骨单个点位多次压缩力测试系统,软骨盘受限压缩测试系统,无侧限压缩仪,无侧限压缩系统

型号:biomomentum mach-1
联系人:李先生
联系电话:18618101725
品牌:biomomentum

多功能组织材料生物力学特性、电位分布测试分析表征系统 
-之侧限压缩测试和无侧限压缩测试

该系统是能集成压缩、张力、剪切、摩擦、扭转和2D/3D压痕、3D轮廓及多力混合耦连测试的一体化微观力学测试装置。能对生物组织、聚合物、凝胶、生物材料、胶囊、粘合剂和食品进行精密可靠的机械刺激和表征。允许表征的机械性能包括刚度、强度、模量、粘弹性、塑性、硬度、附着力、肿胀和松弛位移控制运动。

特点

1、适用样品范围广:

1.1、从骨等硬组织材料到脑组织、眼角膜等软组织材料

1.2、从粗椎间盘的样品到j细纤维丝

2、通高量压痕测试分析

◆无需表面平坦,可在不规则表面压痕
◆压痕同时可测量厚度信息
◆压痕不要求压缩轴垂直于样品表面对齐
◆红宝石压头,坚固不易断
◆样品不需要从组织中收集
◆组织的破坏小
◆维持被测材料的机械环境及其与周围材料的相互作用
◆测试多个站点mapping


2.1、三维法向压痕映射非平面样品整个表面的力学特性

2.2、48孔板中压痕测试分析

3、力学类型测试分析功能齐

模块化集成压缩、张力、剪切、摩擦、扭转、穿刺、摩擦和2D/3D压痕、3D表面轮廓、3D厚度等各种力学类型支持,微观结构表征及动态力学分析研究

4、高分辨率:

4.1、位移分辨率达0.1um

4.2、力分辨率 达0.025mN

5、 行程范围广:50-250mm

6、体积小巧、可放入培养箱内

7 、高变分辨率成像跟踪分析

8、多轴向、多力偶联刺激

9、活性组织电位分布测试分析

10、产品成熟,文献量达 上千篇

侧限压缩(confined compression):受压样品的周围有限制,受压过程中除垂直方向变形外,不会侧向膨胀变形。 ,通常在双相材料(一相为液体)(例如多孔弹性材料)上进行密闭压缩测试。 该测试通常在密闭的圆柱形腔室中进行,其中样品完充满了腔室的体积。 活塞用于向样品施加应力。 在液相不可压缩的情况下,密闭压缩室的至少一个壁必须是多孔的,以使材料变形。 当流体停止流过时,总模量(Ha)对应于处于平衡状态的材料的刚度。
CONFINED COMPRESSION
Confined compression testing measures the capacity of a material to withstand axial compressive loads without expanding perpendicularly to the force. Confined compression tests are usually performed on biphasic materials (one phase being liquid) (e.g. poroelastic materials). This test is generally conducted in a closed cylindrical chamber where the sample completely fills the chamber volume. A piston is used to apply stress to the sample. With the liquid phase being incompressible, at least one wall of the confined compression chamber must be porous to deform the material. The aggregate modulus (Ha) corresponds to the stiffness of the material at equilibrium when fluid has ceased flowing through it.

MA742密闭腔室套件,直径3毫米(用于MA626)

腔室由透明管制成(内径D = 3.2 mm),可在压缩过程中观察样品。 圆盘状样品被滑动活塞压缩,

液相可通过多孔盘(孔径为40微米)排出。

材质:不锈钢316L(多孔盘,孔径40微米)

直径:3.2毫米(内径)

MACH-1分析软件-附加的无限制和无限制压缩(弹性)

该工具可以计算和可视化总模量(MPa),侧限压缩中样品的“应力松弛”曲线(毫米或力与时间的关系)计算出的

渗透率(mm2 / MPa·s)和拟合的均方误差(MSE)。

将曲线拟合到线性双相模型(Mow等,1980或Bursa等,1999),并引入圆盘的机械性能。

该模型非常适合用于容纳液体部分的样品的机械和描述的固体基质模型,为了获得有限压缩中的多孔弹性模型的结果。

MACH-1 ANALYSIS SOFTWARE - ADD-ON FOR UNCONFINED & CONFINED COMPRESSION (POROELASTIC)

MA726

This tool allows for the computing and visualizing of the aggregate modulus (MPa), the permeability (mm2/MPa·s) and the mean square error (MSE) of the fit calculated from a “Stress Relaxation” curve (load or force vs. time) in confined compression on a disk sample. The curve is fitted to the linear biphasic model (Mow et al., 1980 or Bursa? et al., 1999) in order to obtain the mechanical properties of the disk. Sample radius, strain and sample thickness need to be specified. This model is well adapted model for the mechanical description of samples containing a liquid fraction and a solid matrix, for example: cartilage or gel samples. A user license is required in order to obtain the results of the poroelastic model in confined compression (Software Add-On, contact Biomomentum to obtain your license).

 



侧限压缩仪测试可测量材料承受轴向压缩负载而不会垂直于力膨胀的能力。通常在双相材料(一相为液体)(例如多孔弹性材料)上进行密闭压缩测试。该测试通常在密闭的圆柱形腔室中进行,其中样品完充满了腔室的体积。活塞用于向样品施加应力。在液相不可压缩的情况下,密闭压缩室的至少一个壁必须是多孔的,以使材料变形。当流体停止流过时,总模量(Ha)对应于处于平衡状态的材料的刚度。

Mach-1分析-受限压缩中的多孔弹性模型-示例(Mach-1 Analysis - Poroelastic Model in Confined Compression - Example)


Mach-1分析-有限压缩中的多孔弹性模型-参数(Mach-1 Analysis - Poroelastic Model in Confined Compression - Parameters)


侧限压缩相关文献:



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While multiple types of smart, environmentally-responsive materials have been explored for a variety of biomedical applications (e.g. drug delivery, tissue engineering, bioimaging, etc.), their ultimate clinical use has been hampered by their lack of biologically-relevant degradation as well as challenges regarding their non-surgical administration to the body. These factors have particularly limited...Read More
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Introduction: Scaffolds are among the key components of a tissue-engineered construct. Although many different proteins have been used in scaffold preparation, gelatin, which is obtained by degradation of collagen, is a completely biodegradable, highly compatible, and low-cost raw material. These characteristics justify its success in numerous applications in tissue engineering and other fields...Read More
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Information on stent longitudinal strength is limited to benchtop models using unconstrained stents, consequently having uncertain clinical significance. This study investigated the effects of stent apposition and tube (artery) compliance on stent stability. Multilink Vision, Multilink 8, Promus Element, Driver and Integrity were examined. Stent size was 3/18 mm or closest length. Four scenarios were...Read More
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SUMMARY OF BACKGROUND DATA: There...Read More
Indentation Method to Map Mechanical Properties of Articular Surface to Identify Degenerated RegionsSim S, Chevrier A, Garon M, Quenneville E and Buschmann MD2014 Annual Meeting of the Biomedical Engineering Society, 2014, San Antonio, TX, USA. Podium presentation (ID: OP-Sat-3-4)
Introduction: The identification and quantitative grading of early degenerated regions over an entire articular surface remains a challenging quest. The objective of this study was to investigate the ability of a novel technique to automatically characterize mechanical properties of entire human articular surfaces in indentation in order to rapidly and non-destructively discriminate between...Read More
Correlation of Non-destructive Electromechanical Probe (Arthro-BST) Assessment with Histological Scores and Mechanical Properties in Human Tibial PlateauSim S, Chevrier A, Garon M, Quenneville E and Buschmann MDOrthopeadic Research Society Annual Meeting in Las Vegas, 2015, Poster 1228
The purpose of the study was to investigate if electromechanical properties of human tibial plateau correlate strongly with histological scores and with biomechanical properties as in human distal femurs (Sim et al., 2014). Six pairs of tibial plateau from human donors (5 males and 1 female, average age 48 years) were provided by RTI Surgical (FL, USA). Ex vivo electromechanical properties were mapped...Read More
Evaluation of Entire Ovine Cartilage Repair Articular Surfaces: Mechanical and Electromechanical AssessmentSim S, Hadjab I, Garon M, Quenneville E, Hurtig MB, Buschmann MD and Hoemann CDTransactions of International Cartilage Repair Society (ICRS), Chicago, 2015, 7-11 May 2015, e-Poster: P87
Purpose: To demonstrate the ability of non-destructive electromechanical device and automated indentation technique in assessing the quality of cartilage in a sheep model of cartilage repair. 

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In a recent study, our group has highlighted the importance of considering the natural topographic variability of the mechanical properties over the articular surface, particularly in the context of cartilage repair, where it can screen the effect of a treatment [1]. Moreover, the availability of test sample is limited in those repair studies since the regions of interest are often limited in size....Read More
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This study mainly evaluates the elastic modulus of 316 stainless steel lattice structures fabricated via binder jetting process. In this present research, both solid and lattice samples are designed and fabricated by binder jetting process for two different types of mechanical tests. Besides experimental study, a numerical model based on energy approach has been proposed to predict the effective elastic...Read More
Experimental Evidence of Mechanical Isotropy in Porcine Lung ParenchymaWeed B, Patnaik S, Rougeau-Browning M, Brazile B, Liao J, Prabhu R and Williams LNMaterials 2015, 8 , 2454-2466; doi:10.3390/ma8052454
Pulmonary injuries are a major source of morbidity and mortality associated with trauma. Trauma includes injuries associated with accidents and falls as well as blast injuries caused by explosives. The prevalence and mortality of these injuries has made research of pulmonary injury a major priority. Lungs have a complex structure, with multiple types of tissues necessary to allow successful respiration....Read More
Injectable Interpenetrating Network Hydrogels via Kinetically Orthogonal Reactive Mixing of Functionalized Polymeric PrecursorsTrevor G, Niels M. B. Smeets and Hoare TACS Macro Letters, September 16, 2015, 1104–9. doi:10.1021/acsmacrolett.5b00362
The enhanced mechanics, unique chemistries, and potential for domain formation in interpenetrating network (IPN) hydrogels have attracted significant interest in the context of biomedical applications. However, conventional IPNs are not directly injectable in a biological context, limiting their potential utility in such applications. Herein, we report a fully injectable and thermoresponsive interpenetrating...Read More
Stochastic resonance is a method to improve the biosynthetic response of chondrocytes to mechanical stimulationWeber JF and Waldman SDJournal of Orthopaedic Research 08/2015; DOI:10.1002/jor.23000
Cellular mechanosensitivity is an important factor during the mechanical stimulation of tissue engineered cartilage. While the application of mechanical stimuli improves tissue growth and properties, chondrocytes also rapidly desensitize under prolonged loading thereby limiting its effectiveness. One potential method to mitigate load-induced desensitization is by superimposing noise on the loading...Read More
Direct reactive electrospinning of degradable hydrogel nanofibers for tissue engineeringXu F, Gough I and Hoare TOral #520.5 on Sunday, May 22 from 14:00 to 16:00 in room 516C during World Biomaterials Congress, Montreal, May 2016.
Hydrogels have received considerable attention in the context of soft tissue engineering due to their as high water contents, (general) cell compatibility, and physicochemical and mechanical similarities to native soft tissues[1]. Most hydrogels used for such applications are either monolithic or macroporous with primarily spherical pore structures, despite the fact that it is known that native extracellular...Read More
Experimental Investigation of Mechanical and Thermal Properties of Silica Nanoparticle-Reinforced Poly(acrylamide) Nanocomposite HydrogelsZaragoza J, Babhadiashar N, O’Brien V, Chang A, Blanco M, Zabalegui A, H Lee and Asuri PPLoS ONE 10(8): e0136293. doi:10.1371/journal. pone.0136293. (2015)
Current studies investigating properties of nanoparticle-reinforced polymers have shown that nanocomposites often exhibit improved properties compared to neat polymers. However, over two decades of research, using both experimental studies and modeling analyses, has not fully elucidated the mechanistic underpinnings behind these enhancements. Moreover, few studies have focused on developing an understanding...Read More
Infarcted rat myocardium: Data from biaxial tensile and uniaxial compressive testing and analysis of collagen fibre orientationSirry MS, Butler JR, Patnaik SS, Brazile B, Bertucci R, Claude A, McLaughlin R, Davies NH and Liao JData in Brief 8 (2016) 1338–1343
Myocardial infarction was experimentally induced in rat hearts and harvested immediately, 7, 14 and 28 days after the infarction induction. Anterior wall infarct samples underwent biaxial tensile and uniaxial compressive testing. Orientation of collagen fibres was analysed following mechanical testing. In this paper, we present the tensile and compressive stress–strain raw data, the calculated tensile...Read More
Correlation of Non-destructive Electromechanical Probe (Arthro-BST) Assessment with Histological Scores, Biochemical Composition and Mechanical Properties in Human Knee JointsSim S, Chevrier A, Quenneville E, Garon M and Buschmann MDTransactions of the 60th Annual Meeting of the Orthopaedic Research Society, New Orleans, LA, USA, Poster 0439, 2014
Introduction: Histological scoring, biochemical analyses and biomechanical testing (unconfined compression) are often seen as gold standard characterizations for articular cartilage but can present major drawbacks in the context of animal and human studies where characterization of complete intact articular cartilage surfaces is required. In particular, histology, biochemical and mechanical...Read More
Novel Technique to Map the Biomechanical Properties of Entire Articular Surfaces Using Indentation to Identify Osteoarthritis-like RegionsSim S, Chevrier A, Garon M, Quenneville E and Buschmann MDTransactions of the 60th Annual Meeting of the Orthopaedic Research Society, 2014, New Orleans, LA, USA, 2015, Poster #2015
Introduction: It is challenging to identify and grade degenerated regions of the entire articular surface both quantitatively and non-destructively. Therefore, the objective of this study was to investigate the ability of a novel technique to automatically characterize mechanical properties of entire articular surfaces in indentation to rapidly discriminate between damaged articular cartilage...Read More
A composite hydrogel for brain tissue phantomsForte AE, Galvan S, Manieri F,y Baena FR and Dini DMaterials & Design, 112, 227-238 (2016)
Synthetic phantoms are valuable tools for training, research and development in traditional and computer aided surgery, but complex organs, such as the brain, are difficult to replicate. Here, we present the development of a new composite hydrogel capable of mimicking the mechanical response of brain tissue under loading. Our results demonstrate how the combination of two different hydrogels, whose...Read More
Biomechanical Characterisation of the Human Auricular Cartilage - Implications for Tissue EngineeringGriffin MF, Premakumar Y, Seifalian M, Szarko and Butler PEMAnnals of Biomedical Engineering, 2016. Punlished online, 14Jul2016. DOI: 10.1007/s10439-016-1688-1
Currently, autologous cartilage provides the gold standard for auricular reconstruction. However, synthetic biomaterials offer a number of advantages for ear reconstruction including decreased donor site morbidity and earlier surgery. Critical to implant success is the material’s mechanical properties as this affects biocompatibility and extrusion. The aim of this study was to determine the biomechanical...Read More
Streaming Potential-Based Arthroscopic Device Discerns Topographical Differences In Cartilage Covered And Uncovered By Meniscus In Ovine Stifle JointsChangoor A, Quenneville E, Garon M, Cloutier L, Hurtig MB and Buschmann MDTransactions of the 53th Annual Meeting of the Orthopaedic Research Society, San Diego, CA, USA, 32:631. (2007)
Animal models of osteoarthritis have been used for understanding diseaseprogression and are essential for assessing potential new therapies. Ovine models, such as the lateral meniscectomy model, are of interest because meniscectomy models often follow a disease progression similar to that in humans, and joint size is sufficient for multiple analyses of cartilage including biomechanical, biochemical...Read More
Streaming Potential-Based Arthroscopic Device Can Detect Changes Immediately Following Localized Impact in an Equine Impact Model of OsteoarthritisChangoor A, Quenneville E, Garon M, Hurtig MB and Buschmann MDOsteoarthritis and Cartilage, Vol. 17, Supplement 1, S53, World Congress on Osteoarthritis, September 2009, Montreal, Quebec, Canada. (2009)
Early Post-traumatic Osteoarthritis (PTOA) can be asymptomatic but represents a possible window of opportunity for therapeutic intervention before disease progression. Impact models are ideal for studying these strategies because the location and severity of impact can be controlled. Streaming potentials generated during cartilage compression are a sensitive measure of degeneration, which may be valuable...Read More
ASTM D1621 - Standard Test Method for Compressive Properties of Rigid Cellular PlasticsASTM International, West Conshohocken, PA, 2016, www.astm.org
Significance and Use

4.1 This test method provides information regarding the behavior of cellular materials under compressive loads. Test data is obtained, and from a complete load-deformation curve it is possible to compute the compressive stress at any load (such as compressive stress at proportional-limit load or compressive strength at maximum load) and to compute the effective...Read More
ASTM D3574 - Standard Test Methods for Flexible Cellular Materials—Slab, Bonded, and Molded Urethane FoamsASTM International, West Conshohocken, PA, 2011, www.astm.org
Significance and Use

The test procedures provide a standard method of obtaining data for research and development, quality control, acceptance and rejection under specifications, and special purposes.
The data obtained by these test methods are applicable to the material under conditions of the particular test and are not necessarily the same as obtained in other environments...Read More
ASTM D575 - Standard Test Methods for Rubber Properties in CompressionASTM International, West Conshohocken, PA, 2012, www.astm.org
Significance and Use

These test methods are useful in comparing stiffness of rubber materials in compression. They can be used by rubber technologists to aid in development of materials for compressive applications.

Scope

1.1 These test methods cover two test procedures for determining the compression-deflection characteristics...Read More
ASTM E132 - Standard Test Method for Poisson's Ratio at Room TemperatureASTM International, West Conshohocken, PA, 2010, www.astm.org
Significance and Use

When uniaxial force is applied to a solid, it deforms in the direction of the applied force, but also expands or contracts laterally depending on whether the force is tensile or compressive. If the solid is homogeneous and isotropic, and the material remains elastic under the action of the applied force, the lateral strain bears a constant relationship to...Read More
ASTM F1538 - Standard Specification for Glass and Glass Ceramic Biomaterials for ImplantationASTM International, West Conshohocken, PA, 2009, www.astm.org
Abstract

This specification covers the material requirements and characterization techniques for glass and glass-ceramic biomaterials intended for use as bulk porous or powdered surgical implants, or as coatings on surgical devices, but not including drug delivery systems. Glass and glass-ceramic biomaterials should be evaluated thoroughly for biocompatibility before human use....Read More
ASTM F1717 - Standard Test Methods for Spinal Implant Constructs in a Vertebrectomy ModelASTM International, West Conshohocken, PA, 2015, www.astm.org
Significance and Use

5.1 Spinal implants are generally composed of several components which, when connected together, form a spinal implant assembly. Spinal implant assemblies are designed to provide some stability to the spine while arthrodesis takes place. These test methods outline standard materials and methods for the evaluation of different spinal implant assemblies so...Read More
ASTM F2077 - Test Methods For Intervertebral Body Fusion DevicesASTM International, West Conshohocken, PA, 2014, www.astm.org
Significance and Use

5.1 Intervertebral body fusion device assemblies are generally simple geometric-shaped devices which are often porous or hollow in nature. Their function is to support the anterior column of the spine to facilitate arthrodesis of the motion segment. This test method outlines materials and methods for the characterization and evaluation of the mechanical performance...Read More
ASTM F2451-05 - Standard Guide for in vivo Assessment of Implantable Devices Intended to Repair or Regenerate Articular CartilageASTM International, West Conshohocken, PA, 2010, www.astm.org
Significance and Use

This guide is aimed at providing a range of in vivo models to aid in preclinical research and development of tissue engineered medical products intended for the clinical repair or regeneration of articular cartilage.
This guide includes a description of the animal models, surgical considerations, and tissue processing as well as the qualitative and quantitative...Read More
Mach-1 - Unconfined Compression of an Articular Cartilage Osteochondral Core (MA056-SOP03-D v2)Sim S and Quenneville EBiomomentum Inc. Laval (QC), Canada, Effective Date: April 7th, 2015
Purpose: This procedure describes a standard method to realize unconfined compression on an osteochondral core using the Mach-1 mechanical tester.

Scope: This procedure can be used for the ex vivo unconfined compression on an osteochondral core. It is highly recommended to use standard protocol for sample preparation to facilitate positioning...Read More
Mach-1 - Unconfined Compression of a Disk (MA056-SOP05-D v1)Sim S and Quenneville EBiomomentum Inc. Laval (QC), Canada, Effective Date: April 14th, 2015
Purpose 
This procedure describes a standard method to realize unconfined compression on a disk-shaped sample using the Mach-1TM mechanical tester. 

Scope 
This procedure can be used for the unconfined compression of a disk-shaped sample. It is highly recommended to use standard protocol for sample preparation to facilitate positioning...Read More
Mach-1 Analysis - Extraction of Mechanical Parameters Following Unconfined Compression (SW186-SOP03-D v1)Sim SBiomomentum Inc. Laval (QC), Canada, Effective Date: April 15th, 2015
Purpose 
This procedure describes a method to extract mechanical parameters from Mach-1 result files generated during an unconfined compression of a sample (e.g. an osteochondral core (as per MA056-SOP03-D) or a disk (as per MA056-SOP05-D)). It also describes the creation of corresponding “.map” characterization file.  
Scope 
This procedure can...Read More
Tailoring hydrogel surface properties to modulate cellular response to shear loadingMeinert C, Schrobback K, Levett PA, Lutton C, Sah R and Klein TJActa biomaterialia, October 2016. DOI: 10.1016/j.actbio.2016.10.011
Biological tissues at articulating surfaces, such as articular cartilage, typically have remarkable low-friction properties that limit tissue shear during movement. However, these frictional properties change with trauma, aging, and disease, resulting in an altered mechanical state within the tissues. Yet, it remains unclear how these surface changes affect the behaviour of embedded cells when the...Read More
Biomechanical characterisation of the human nasal cartilages - implications for tissue engineeringGriffin MF, Premakumar Y, Seifalian AM, Szarko M and Butler PEMJournal of Materials Science: Materials in Medicine, 27(1), 1-6. (2016)
Nasal reconstruction is currently performed using autologous grafts provides but is limited by donor site morbidity, tissue availability and potentially graft failure. Additionally, current alternative alloplastic materials are limited by their high extrusion and infection rates. Matching mechanical properties of synthetic materials to the native tissue they are replacing has shown to be important...Read More
Change in viability of C2C12 myoblasts under compression, shear and oxidative challengesHong Y, Yao Y, Wong S, Bian L and Mak AFJournal of biomechanics, 49(8), 1305-1310. (2016)
Skeletal and epidermal loadings can damage muscle cells and contribute to the development of deep tissue injury (DTI) – a severe kind of pressure ulcers affecting many people with disability. Important predisposing factors include the multiaxial stress and strain fields in the internal tissues, particularly the vulnerable muscles around bony prominences. A careful study of the mechanical damage thresholds...Read More
A fibril-network-reinforced biphasic model of cartilage in unconfined compressionSoulhat J, Buschmann MD and Shirazi-Adl AJournal of Biomechanical Engineering, Jun, 121(3), 340-347. (1999)
Cartilage mechanical function relies on a composite structure of a collagen fibrillar network entrapping a proteoglycan matrix. Previous biphasic or poroelastic models of this tissue, which have approximated its composite structure using a homogeneous solid phase, have experienced difficulties in describing measured material responses. Progress to date in resolving these difficulties has demonstrated...Read More
ISO 4049 - Dentistry - Polymer-based restorative materialsISO
This International Standard specifies requirements for dental polymer-based filling and restorative materials and polymer-based luting materials supplied in a form suitable for mechanical mixing, hand-mixing, or intra-oral and extra-oral external energy activation, and intended for use primarily for the direct or indirect restoration of cavities in the teeth. The following section of the standard...Read More
Characterization of tissue engineered cartilage products - Recent developments in advanced therapyMaciulaitis J, Rekstyte S, Usas A, Jankauskaite V, Gudas R, Malinauskas M and Maciulaitis RPharmacological Research 113 (2016) 823–832
Legislative requirements for the quality of pharmacological agents underwent certain evolution when new type of therapies emerged. This relates to cell based medicines, such as tissue engineered cartilage products (TECP) which are increasingly developed as new modalities for widely prevalent orthopaedic disorders. Although quality measures for TECP are subject to the same general regulatory quality...Read More
Review Paper: Fifty years of brain tissue mechanical testing: From in vitro to in vivo investigationsChatelin S, Constantinesco A and Willinger RBiorheology 47 (2010) 255–276. DOI 10.3233/BIR-2010-0576
Beginning in the 1960s many studies have been performed to investigate the mechanical properties of brain. In this paper we point out the difficulties linked with in vitro experimental protocols as well as the advantages of using recently developed non-invasive in vivo techniques, such as magnetic resonance elastography. Results of in vitro and in vivo work are compared, emphasizing the specificities...Read More
Combined Mechanical Characterizations Increases Sensitivity in the Assessment of Human Cartilage DegenerationSim S, Hadjab I, Chevrolat L-A, Masse M, Tong AL, Lavigne P, Garon M, Quenneville E and Buschmann MDAccepted for a podium presentation at ORS 2017
Introduction: We published a recent study showing superior sensitivity of electromechanical and indentation (instantaneous response) assessments versus well-established techniques, including histological Mankin score, to characterize cartilage degeneration. This study aims to determine whether the combination of instantaneous, relaxation and equilibrium mechanical properties and friction...Read More
Evaluation of genipin for stabilization of decellularized porcine cartilageElder S, Pinheiro A, Young C, Smith P and Wright EJ Orthop Res. 2016 Nov 18. doi: 10.1002/jor.23483
Abstract
We speculate that an acellular osteochondral xenograft may be a good alternative to allografts for repair of focal articular cartilage lesions. In order to make a xenograft resistant to enzymatic degradation and to prevent a chronic immune response it may beneficial to stabilize it through crosslinking. The concept is analogous to treatment of porcine bioprosthetic heart...Read More
Strengthening of C2C12 mouse myoblasts against compression damage by mild cyclic compressive stimulationYao Y and Mak AFJ Biomech. 2016 Dec 8;49(16):3956-3961. doi: 10.1016/j.jbiomech.2016.11.050. Epub 2016 Nov 16
Abstract
Deep tissue injury (DTI) is a severe kind of pressure ulcers formed by sustained deformation of muscle tissues over bony prominences. As a major clinical issue, DTI affects people with physical disabilities, and is obviously related to the load-bearing capacity of muscle cells in various in-vivo conditions. It is important to provide a preventive approach to help muscle cells...Read More
Biomechanical Characterization of Human Soft Tissues Using Indentation and Tensile TestingGriffin M, Premakumar Y, Seifalian A, Butler PE and Szarko MJournal of Visualized Experiments◆: JoVE. 2016;(118):54872. doi:10.3791/54872.
Abstract
Regenerative medicine aims to engineer materials to replace or restore damaged or diseased organs. The mechanical properties of such materials should mimic the human tissues they are aiming to replace; to provide the required anatomical shape, the materials must be able to sustain the mechanical forces they will experience when implanted at the defect site. Although the...Read More
Cryogenic 3D Printing of Super Soft HydrogelsForte AE, Parisi C, Dini D, Di Silvio L and Tan ZScientific Reports 7, Article number: 16293 (2017) doi:10.1038/s41598-017-16668-9
Conventional 3D bioprinting allows fabrication of 3D scaffolds for biomedical applications. In this contribution we present a cryogenic 3D printing method able to produce stable 3D structures by utilising the liquid to solid phase change of a composite hydrogel (CH) ink. This is achieved by rapidly cooling the ink solution below its freezing point using solid carbon dioxide (CO2) in an isopropanol...Read More
Changes in growth plate extracellular matrix composition and biomechanics following in vitro static versus dynamic mechanical modulationKaviani R, Londono I, Parent S, Moldovan F and Villemure IJ Musculoskelet Neuronal Interact, 1-11 (2017)
The objective of this study was to investigate the effects of mechanical modulation parameters on structural proteins biocomposition and mechanical properties of the growth plate. Establishing these parameters is a crucial step in the development of fusionless treatment of scoliosis. In this study, ulna explants from 4-weeks-old (pubertal) swines were used. The biocomposition was characterized...Read More
Mechanically resilient injectable scaffolds for intramuscular stem cell delivery and cytokine releaseYoung SA, Sherman SE, Cooper TT, Brown C, Anjum F, Hess DA. Flynn LE and Amsden BGBiomaterials. doi: https://doi.org/10.1016/j.biomaterials.2018.01.008 (2018)
A promising strategy for treating peripheral ischemia involves the delivery of stem cells to promote angiogenesis through paracrine signaling. Treatment success depends on cell localization, retention, and survival within the mechanically dynamic intramuscular (IM) environment. Herein we describe an injectable, in situ-gelling hydrogel for the IM delivery of adipose-derived stem/stromal cells (ASCs),...Read More
Quantitative Analysis of Tissue Damage Evolution in Porcine Liver With Interrupted Mechanical Testing Under Tension, Compression, and Shear.Chen J, Brazile B, Prabhu R, Patnaik SS, Bertucci R, Rhee H, Horstemeyer MF, Hong Y, Williams LN, Liao JJ Biomech Eng. 2018 Jul 1;140(7). doi: 10.1115/1.4039825.
In this study, the damage evolution of liver tissue was quantified at the microstructural level under tensile, compression, and shear loading conditions using an interrupted mechanical testing method. To capture the internal microstructural changes in response to global deformation,...Read More
Addition of excess thyroid hormone induces detrimental changes in human ex vivo full thickness osteochondral explantsHoutman, E.; van Hoolwerff, M.; Ruiz, A. Rodriguez; Lakenberg, N.; Suchiman, E.; Nelissen, R.; Ramos, Y.; Meulenbelt, I.Osteoarthritis and Cartilage 26 (2018) S400 https://doi.org/10.1016/j.joca.2018.02.777
Purpose: By applying 3D in vitro chondrogenesis in a model using human bone marrow mesenchymal stem cells (hBMSC), it was demonstrated that excess thyroid hormone (T3) and the upregulation of deiodinase iodothyronine type-2 (D2) gene (DIO2), had similar detrimental effects on cartilage matrix deposition. Moreover, an in vivo rat model further indicated that this unfavourable effect of DIO2 upregulation...Read More
Development of a Breast Cancer Cell Microenvironment in Three-Dimensional Hyaluronic Acid Based HydrogelsFisher, Stephanie Anne.Thesis, University of Toronto. (2018)
Breast cancer cell invasion is influenced by the tumor microenvironment including the extracellular matrix, mechanical properties, stromal cells, and bioactive factors. To elucidate the role of the microenvironment on cell invasion, defined 3-dimensional hydrogel models are required because xenograft models are too complex and 2-dimensional models lack a matrix for cell invasion and often do not recapitulate...Read More
Effects of bone damage on creep behaviours of human vertebral trabeculaeP OCallaghan, M Szarko, Y Wang and J LuoBone Volume 106, January 2018, Pages 204-210
A subgroup of patients suffering with vertebral fractures can develop progressive spinal deformities over time. The mechanism underlying such clinical observation, however, remains unknown. Previous studies suggested that creep deformation of the vertebral trabeculae may play a role. Using the acoustic emission (AE) technique, this study investigated effects of bone damage (modulus reduction) on creep...Read More
Fibronectin promotes elastin deposition, elasticity and mechanical strength in cellularised collagen-based scaffoldsPezzoli, Daniele; Di Paolo, Joseph; Kumra, Heena; Fois, Giulia; Candiani, Gabriele; Reinhardt, Dieter P.; Mantovani, DiegoBiomaterials Volume 180, October 2018, Pages 130-142 https://doi.org/10.1016/j.biomaterials.2018.07.013
One of the tightest bottlenecks in vascular tissue engineering (vTE) is the lack of strength and elasticity of engineered vascular wall models caused by limited elastic fiber deposition. In this study, flat and tubular collagen gel-based scaffolds were cellularised with vascular smooth muscle cells (SMCs) and supplemented with human plasma fibronectin (FN), a known master organizer of several extracellular...Read More
In Situ Cross-Linking of Poly (vinyl alcohol)/Graphene Oxide–Polyethylene Glycol Nanocomposite Hydrogels as Artificial Cartilage Replacement: Intercalation Structure, Unconfined Compressive Behavior, and Biotribological BehaviorsMeng, Yeqiao; Ye, Lin; Coates, Phil; Twigg, PeterJ. Phys. Chem. C, 2018, 122 (5), pp 3157–3167 DOI: 10.1021/acs.jpcc.7b12465
Poly(vinyl alcohol) (PVA)/graphene oxide (GO) nanocomposite hydrogel as artificial cartilage replacement was prepared via freezing/thawing method by introducing polyethylene glycol (PEG). Efficient grafting of PVA molecules onto GO surface was realized by formation of hydrogen bonding, resulting in exfoliation and uniform distribution of GO in PVA matrix. By introduction of appropriate content of GO,...Read More
Mechanically enhanced nested-network hydrogels as a coating material for biomedical devicesWang, Zhengmu; Zhang, Hongbin; Chu, Axel J.; Jackson, John; Lin, Karen; Lim, Chinten James; Lange, Dirk; Chiao, MuActa Biomaterialia Volume 70, 1 April 2018, Pages 98-109 https://doi.org/10.1016/j.actbio.2018.02.003
Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms. Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl...Read More
Models and tissue mimics for brain shift simulationsForte, Antonio E.; Galvan, Stefano; Dini, DanieleBiomech Model Mechanobiol (2018) 17: 249. https://doi.org/10.1007/s10237-017-0958-7
Capturing the deformation of human brain during neurosurgical operations is an extremely important task to improve the accuracy or surgical procedure and minimize permanent damage in patients. This study focuses on the development of an accurate numerical model for the prediction of brain shift during surgical procedures and employs a tissue mimic recently developed to capture the complexity of the...Read More
Morphology of cross-linked cellulose nanocrystal aerogels: cryo-templating versus pressurized gas expansion processingOsorio, Daniel A.; Seifried, Bernhard; Moquin, Paul; Grandfield, Kathryn; Cranston, Emily D.Journal of Materials Science July 2018, Volume 53, Issue 13, pp 9842–9860
Cellulose nanocrystal (CNC)-based aerogels are often produced through cryo-templating, followed by either critical point drying or freeze drying. While cryo-templating gives aerogels with a bimodal pore size distribution, better morphological control may be needed for certain applications. This work compares CNC aerogels prepared using a new processing method, called pressurized gas expansion (PGX)...Read More
Nanocolloidal Hydrogel for Heavy Metal ScavengingAlizadehgiashi, Moien; Khuu, Nancy; Khabibullin, Amir; Henry, Andria; Tebbe, Moritz; Suzuki, Toyoko; Kumacheva, EugeniaACS Nano, Article ASAP DOI: 10.1021/acsnano.8b03202 Publication Date (Web): July 6, 2018
We report a nanocolloidal hydrogel that combines the advantages of molecular hydrogels and nanoparticle-based scavengers of heavy metal ions. The hydrogel was formed by the chemical cross-linking of cellulose nanocrystals and graphene quantum dots. Over a range of hydrogel compositions, its structure was changed from lamellar to nanofibrillar, thus enabling the control of hydrogel permeability. Using...Read More
Patterning of Structurally Anisotropic Composite Hydrogel SheetsPrince, Elisabeth; Alizadehgiashi, Moien; Campbell, Melissa; Khuu, Nancy; Albulescu, Alexandra; De France, Kevin; Ratkov, Dimitrije; Li, Yunfeng; Hoare, Todd; Kumacheva, EugeniaBiomacromolecules, 2018, 19 (4), pp 1276–1284 DOI: 10.1021/acs.biomac.8b00100
Compositional and structural patterns play a crucial role in the function of many biological tissues. In the present work, for nanofibrillar hydrogels formed by chemically cross-linked cellulose nanocrystals (CNC) and gelatin, we report a microextrusion-based 3D printing method to generate structurally anisotropic hydrogel sheets with CNCs aligned in the direction of extrusion. We prepared hydrogels...Read More
Tribological evaluation of biomedical polycarbonate urethanes against articular cartilageKanca, Yusuf; Milner, Piers; Dini, Daniele; Amis, Andrew A.Journal of the Mechanical Behavior of Biomedical Materials Volume 82, June 2018, Pages 394-402 https://doi.org/10.1016/j.jmbbm.2018.04.001
This research investigated the in-vitro wear and friction performance of polycarbonate urethane (PCU) 80A as they interact with articular cartilage, using a customised multidirectional pin-on-plate tester. Condyles were articulated against PCU 80A discs (Bionate I and Bionate II) (configuration 1) and the results arising from these tests were compared to those recorded during the sliding of PCU pins...Read More
Tribological properties of PVA/PVP blend hydrogels against articular cartilageKanca, Yusuf; Milner, Piers; Dini, Daniele; Amis, Andrew A.Journal of the Mechanical Behavior of Biomedical Materials Volume 78, February 2018, Pages 36-45 https://doi.org/10.1016/j.jmbbm.2017.10.027
This research investigated in-vitro tribological performance of the articulation of cartilage-on- polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP) blend hydrogels using a custom-designed multi-directional wear rig. The hydrogels were prepared by repeated freezing-thawing cycles at different concentrations and PVA to PVP fractions at a given concentration. PVA/PVP blend hydrogels showed low coefficient...Read More
Growth and Characterization of Multicellular Spheroids in Cellulose Derived Hydrogels as an Artificial Three-Dimensional ScaffoldMelissa CampbellUniversity of Toronto
The Young’s modulus of each hydrogel was performed on a Mach-1 Mechanical tester (Biomomentum Inc., QC) using cyclic compression and parallel plate geometry. The hydrogels for testing were pre-formed into disks measuring 3.25 mm in height by 14 mm in diameter. Compression was executed by applying 20% strain at a rate of 0.03 mm/s in the +z direction. The Young’s modulus of the hydrogels was...Read More
Peptide-modified methacrylated glycol chitosan hydrogels as a cell-viability supporting pro-angiogenic cell delivery platform for human adipose-derived stem/stromal cellsDhillon J, Young SA, Sherman SE, Bell GI, Amsden BG, Hess DA, Flynn LEJ Biomed Mater Res A. 2018 Nov 3. doi: 10.1002/jbm.a.36573.
Cell-based therapies involving the injection of adipose-derived stem/stromal cells (ASCs) within rationally designed biomaterials are a promising approach for stimulating angiogenesis. With this focus, the current work explored the effects of incorporating integrin-binding RGD or...Read More
Multi-modal characterization of polymeric gels to determine the influence of testing method on observed elastic modulusDavid M. Kingsley, Caitlin H.McCleery, Christopher D.L.Johnson, Michael T.K.Bramson, Deniz Rende, Ryan J.Gilbert, David T.CorrJournal of the Mechanical Behavior of Biomedical Materials, available online 10 January 2019, https://doi.org/10.1016/j.jmbbm.2019.01.003
Demand for materials that mechanically replicate native tissue has driven development and characterization of various new biomaterials. However, a consequence of materials and characterization technique diversity is a lack of consensus within the field, with no clear way to compare values measured via different modalities. This likely contributes to the difficulty in replicating findings across the...Read More
Cross-linked Cellulose Nanocrystal Aerogels as Viable Bone Tissue ScaffoldsDaniel A. Osorioa, Bryan E.J. Lee, Jacek M. Kwiecien, Xiaoyue Wang, Iflah Shahid, Ariana L. Hurley, Emily D. Cranston, Kathryn GrandfieldActa Biomaterialia, 2019, https://doi.org/10.1016/j.actbio.2019.01.049
Chemically cross-linked cellulose nanocrystal (CNC) aerogels possess many properties beneficial for bone tissue scaffolding applications. CNCs were extracted using sulfuric acid or phosphoric acid, to produce CNCs with sulfate and phosphate half-ester surface groups, respectively. Hydrazone cross-linked aerogels fabricated from the two types of CNCs were investigated using scanning electron microscopy,...Read More
Intervertebral disc implantR Kandel, JP Santerre, SQ LiUS Patent App. 10195044, 2019
The present inventors have generated the tissues that comprise the intervertebral disc and have established viable protocols and tissue conditions that facilitate integration of all the tissue components into one functional tissue so it can weight bear following implantation. In particular, the inventors have prepared an intervertebral disc implant containing different integrated components (the...Read More
Comparative study of gelatin methacrylate hydrogels from different sources for biofabrication applicationsZongjie Wang, Zhenlin Tian, Fredric Menard and Keekyoung KimBiofabrication, Volume 9, Number 4, Published 21 August 2017 © 2017 IOP Publishing
Gelatin methacrylate (GelMA) hydrogel is a promising bioink for biofabrication applications due to its cost-effectiveness, ease of synthesis and biocompatibility to allow cell adhesion. However, the GelMA synthesized from a widely used porcine skin gelatin has a thermal gelation problem at room temperature. Here, we present thermally stable GelMA hydrogels at room temperature while maintaining the...Read More
Comparative study on complexes formed by chitosan and different polyanions: Potential of chitosan-pectin biomaterials as scaffolds in tissue engineeringFernanda Carla Bombaldi de Souza, Renata Francielle Bombaldi de Souza, Bernard Drouin, Diego Mantovani and Angela Maria MoraesInternational Journal of Biological Macromolecules, Volume 132, 1 July 2019, Pages 178-189
Polyelectrolyte complexes of chitosan (Ch) and pectin (Pc) or alginate (Alg) were produced in the presence or absence of the silicone gel Silpuran® 2130 A/B (Sil) and the surfactant Kolliphor® P188 (Kol). Ch-Pc-Kol-based formulations presented higher porosity (up to 83.3%) and thickness (maximum of 2273.5◆◆m in PBS). Lower water contact angle was observed for Ch-Alg formulations (minimum of...Read More
Injectable Poly(oligoethylene glycol methacrylate)-Based Hydrogels Fabricated from Highly Branched Precursor Polymers: Controlling Gel Properties by Precursor Polymer MorphologyIvan Urosev, Helen Dorrington, Nicola Muzzin, Richard Alsop, Emilia Bakaic, Trevor Gilbert, Maikel Rheinst◆dter and Todd HoareACS Applied Polymer Materials, 2019, 1, 3, 369-380 January 24, 2019 https://doi.org/10.1021/acsapm.8b00085
The physicochemical properties of injectable hydrogels are typically modified by altering the chemistry of the precursor polymer and/or the amount or type of cross-linker, both of which can lead to hydrogels with altered mechanics, swelling, degradation, and other key physical properties. Herein, we describe an alternative approach to tune the properties of injectable hydrogels (here, based on hydrazone...Read More
Morphology and composition play distinct and complementary roles in the tolerance of plantar skin to mechanical loadColin J. Boyle, Magdalena Plotczyk, Sergi Fayos Villalta, Sharad Patel, Shehan Hettiaratchy, Spyros D. Masouros, Marc A. Masen and Claire A. HigginsScience Advances, 09 Oct 2019: Vol. 5, no. 10, eaay0244, DOI: 10.1126/sciadv.aay0244
Plantar skin on the soles of the feet has a distinct morphology and composition that is thought to enhance its tolerance to mechanical loads, although the individual contributions of morphology and composition have never been quantified. Here, we combine multiscale mechanical testing and computational models of load bearing to quantify the mechanical environment of both plantar and nonplantar...Read More
Development of a dispensing based additive manufacturing technique for the creation of alginate bone scatfoldsJ.J. VarunUBC Theses and Dissertations, 2019. https://open.library.ubc.ca/cIRcle/collections/ubctheses/24/items/1.0378434
Bone scaffolds are used to treat large bone defects. There are noted advantages for moving away from the standard of using human bone to create these scaffolds, and instead making them out of synthetic biomaterials. Dispensing Based Additive Manufacturing (DBAM) provides a method for creating these synthetic...Read More
Compressive Mechanical Properties of Porcine Brain: Experimentation and Modeling of the Tissue Hydration EffectsR.K. Prabhu, M.T. Begonia, W.R. Whittington, M.A. Murphy, Y. Mao, J. Liao, L.N. Williams, M.F. Horstemeyer and J. ShengBioengineering 2019, 6, 40; doi:10.3390/bioengineering6020040

Abstract: Designing protective systems for the human head—and, hence, the brain—requires understanding the brain’s microstructural response to mechanical insults. We present the behavior of wet and dry porcine brain undergoing quasi-static and high strain rate mechanical deformations to unravel the effect of hydration on the brain’s biomechanics. Here, native ‘wet’ brain samples...Read More

On-Demand Radial Electrodeposition of Alginate Tubular StructuresD. M. Kingsley, J. A. Capuano and D. T. CorrACS Biomater. Sci. Eng. Publication Date:June 12, 2019, https://doi.org/10.1021/acsbiomaterials.9b00415

We present an electrodeposition technique for fabricating tubular alginate structures. In this technique, two electrodes (anode and cathode) are suspended in a solution of alginate and insoluble calcium carbonate particles, and the application of an electrical potential produces a localized pH change at the anode surface causing suspended divalent cations to become soluble and cross-link the alginate....Read More

High-Throughput Synthesis and Characterization of Aryl Silicones Using the Piers-Rubinsztajn ReactionA.F. Schneider and M.A. BrookChemistry–A European Journal 09 October 2019 https://doi.org/10.1002/chem.201903658

Arylsilicones are widely exploited for their thermal and optical properties. The creation of phenylsilicone elastomers with specific physical properties is typically done by a ‘one off’ formulation and test process. We demonstrate that high◆throughput synthesis methods can be used to rapidly prepare a series of arylsilicone elastomers and then assess the relative impact of different aryl groups...Read More

Hierarchically Structured, Self-Healing, Fluorescent, Bioactive Hydrogels with Self-Organizing Bundles of Phage NanofilamentsA. Peivandi, L. Tian, R. Mahabir and Z. HosseinidoustChem. Mater. 201931155442-5449 Publication Date:July 24, 2019 https://doi.org/10.1021/acs.chemmater.9b00720

Bacteriophages are essentially bionanoparticles with a protein coat, the composition of which can be controlled with atomic precision via genetic engineering, a property that makes them superior to synthetic nanoparticles as building blocks for bottom-up synthesis of multifunctional materials with advanced properties. We report hierarchically structured hydrogels of self-organized M13 bacteriophage...Read More

Properties of Biopolymer Films in Ionic Solutions and Their Applications as 3D Printed Personalized Wound DressingsD.P. RamosUniversity of Toronto (Canada), ProQuest Dissertations Publishing, 2019. 13805509.

This thesis describes the effects of ionic solutions on biopolymer films and the development of a 3D printed personalized wound dressing.

Chitosan, gelatin, and composite chitosan/gelatin films were exposed to NaCl, Na2SO4, and CaCl2 solutions with varying ionic strength and their swelling and dissolution properties were measured. In addition, the release of the small ionic molecules Rhodamine...Read More

Synthetic presentation of noncanonical Wnt5a motif promotes mechanosensing-dependent differentiation of stem cells and regenerationR. Li, S. Lin, M.Zhu, Y. Deng, X. Chen, K. Wei, J. Xu, G. Li and L. BianSci Adv 5, 10, eaaw 3896, DOI: 10.1126/sciadv.aaw3896

Noncanonical Wnt signaling in stem cells is essential to numerous developmental events. However, no prior studies have capitalized on the osteoinductive potential of noncanonical Wnt ligands to functionalize biomaterials in enhancing the osteogenesis and associated skeleton formation. Here, we investigated the efficacy of the functionalization of biomaterials with a synthetic Wnt5a mimetic ligand...Read More

Degree of crosslinking and mechanical properties of crosslinked poly(vinyl alcohol) beads for use in solid-phase organic synthesisM.A. Gauthier, J. Luo, D.Calvet, C. Ni, X.X. Zhu, M. Garon and M.D. BuschmannPolymer, Volume 45, Issue 24, November 2004, Pages 8201-8210 https://doi.org/10.1016/j.polymer.2004.09.055
The limited swellability in polar media of the commonly used polystyrene/divinylbenzene (PS-DVB) support materials for solid-phase organic synthesis has led to the development of novel, highly swellable hydrophilic gels designed for use in aqueous or polar media. Poly(vinyl alcohol) beads crosslinked with epichlorohydrin (PVA-EP) were prepared by a two-step inverse-suspension polymerization method....Read More
A 3D Bioprinted Meniscus Implant Reinforced by Chitosan/Poly(Vinyl Alcohol) FibresE. Kapyla, M. K. Khan, S. Pan, R. Nickmanesh, S. Beyer, J. Ault, J. Hwang, T. Mohamed, and S. Wadsworth30th Annual Conference of the European Society for Biomaterials, IX-OS31-01, #270, 12 September 2019, Dresden, Germany.
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