单细胞高通量高分辨率RNA测序系统

从单个细胞进行高通量，高分辨率的RNA测序。微流体系统将每个细胞与我们的水凝胶珠共同包裹，以实现条形码cDNA的分离合成。
是wei壹的scRNA-Seq平台，与其他现有平台相比，该平台可提供增强的实验控制，更多可操作的信息以及更低的每结果总成本

主要势

业界高的封装率：> 90％，双峰率低
更多可操作的信息：捕获稀有细胞亚群和低丰度，wu偏差的转录本
多功能输入要求：多种细胞类型，大小和数量
每个结果的总体成本低：每个细胞低至5美分
运行更多样品的成本更低
设计实验的灵活性
能够处理具有挑战性的样品
追大化临床样本数据

可定制的凝胶珠

系统亮点

?每次运行捕获数千个细胞
?封装与细胞大小wu关
?套件包括500,000条带条形码的凝胶微珠
芯片和试剂
?每个套件多达可编码40,000个细胞，每个芯片可编码4个样品
?完集成的仪器和摄像机控制软

多种应用

癌症：肿瘤分析
免疫生物学：B细胞和T细胞受体分析
发育生物学：细胞间变异鉴定
新应用开发：scATAC-seq，nonPolyA，scNuclei，靶向性scRNA-seq
大细胞类型：心肌细胞，巨核细胞，神经元
?药物发现：新药的鉴定和验证
药物目标
?干细胞：细胞间差异鉴定
?发育生物学：细胞谱系追踪

简化的工作流程：

Publications

A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte. Plasschaert L. W. et al. Nature 560 377-381 (2018).

Population snapshots predict early haematopoietic and erythroid hierarchies. Tusi B. K. et al. Nature 55554-60 (2018).

Single-Cell Analysis of Experience-Dependent Transcriptomic States in Mouse Visual Cortex. Hrvatin S. et al. Nat Neurosci. 21 120-129 (2018).

Clonal analysis of lineage fate in native hematopoiesis. Rodriguez-Fraticelli A. E. et al. Nature 553 212-216 (2018).

Single-Cell Map of Diverse Immune Phenotypes in the Breast Tumor Microenvironment. Azizi, E. et al. Cell 174 1-16 (2018).

Single-cell gene expression reveals a landscape of regulatory T cell phenotypes shaped by the TCR.Zemmour, D. et al. Nature Immunology 19 291-301 (2018).

Single-cell transcriptomics of the developing lateral geniculate nucleus reveals insights into circuit assembly and refinement. Kalish B. T. et al. PNAS online E1051-E1060 (2018).

Unsupervised trajectory analysis of single-cell RNA-seq and imaging data reveals alternate tuft cell origins in the gut. Herring C. A. et al. Cell Syst. 6 37-51 (2018).

Single-Cell Transcriptomics of a Human Kidney Allograft Biopsy Specimen Defines a Diverse Inflammatory Response. Wu H. et al. J Am Soc Nephrol. 29 2069-2080 (2018).

The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution. Briggs J. A. et al. Science 360(6392) (2018).

Haematopoietic stem and progenitor cells from human pluripotent stem cells. Sugimura R. et al. Nature 545 432-438 (2017).

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single cell resolution using RNA sequencing. Tang Q. et al. J. Exp. Med. 214 2875-2887 (2017).

Estimation of immune cell content in tumour tissue using single-cell RNA-seq data. Schelker M. et al. Nat. Comm. 8 8-12 (2017).

Comparative analysis of kidney organoid and adult human kidney single cell and single nucleus transcriptomes. Wu H. et al. bioRxiv doi: http://dx.doi.org/10.1101/232561. (2017).

Mouse embryonic stem cells can differentiate via multiple paths to the same state. Briggs A. A. et al. eLife 6 1-23 (2017).

Single-cell barcoding and sequencing using droplet microfluidics. Zilionis R. et al. Nature Protocols 12 44-73 (2017)

A Single-Cell Transcriptomic Map of the Human and Mouse Pancreas Reveals Inter- and Intra-cell Population Structure. [Epub ahead of print] PMID: 27667365
Baron M, Veres A, Wolock SL, Faust AL, Gaujoux R, Vetere A, Ryu JH, Wagner BK, Shen-Orr SS, Klein AM, Melton DA, Yanai I.;
Cell Syst. 2016 Sep 21. pii: S2405-4712(16)30266-6. doi:
10.1016/j.cels.2016.08.011.

End Sequence Analysis Toolkit (ESAT) expands the extractable information from single-cell RNA-seq data.
Derr et al.
2016, Genome Research 26(10): 1397-1410. Epub 2016 Jul 28

Droplet Barcoding For Single-Cell Transcriptomics Applied To Embryonic Stem Cells
Klein et al.
2015, Cell 161, 1187-1201

Marrying Microfluidics and Barcoding Technology
Cell, May 21, 2015, 161, Issue 5