产品说明
用于膜片钳研究的多通道灌流给药系统
次级离子通道前沿的研究
用于膜片钳研究的多通道灌流给药系统
The Dynaflow® Resolve 设备是您现有膜片钳设置的配件,能够以的速度,控制和灵活性提供解决方案交换。 该系统可以记录任何离子通道电流,使用任何细胞类型,任何化合物和任何实验。
优势
真正的多功能性 适用于任何细胞类型,任何膜片钳配置以及任何类型的离子通道,可以进行从从单细胞通道记录到二次筛选的多方面研究。 复杂的灌流设计方案
快速溶液交换(低毫秒范围)能够达到高效地分析快速动力学。
低溶液的消耗并且可重复使用的芯片。
可以达到消耗150 μl的长时间实验,大限度地减少化学溶液和缓冲液的消耗。
可以达到大优化,有效的安quan药理学
可以进行长时间的实验和长时间给药。 针对粘性化合物进行了优化设计,并设计适用于累积剂量反应。
大化的数据质量
每芯片可以操作大量的细胞。 每个芯片多个化学物质分析。 对于每个化合物可以达到wanquan剂量反应。 Gigaseal 可以记录达到卓越的实验控制。
设备
该设备包括一个带控制器和操纵杆的电动扫描平台,驱动微流控芯片流动的注射泵以及 Dynaflow® Commander 软件。 它可以与大多数倒置显微镜合用。
1 – Dynaflow® Resolve 芯片
Dynaflow® Resolve 芯片具有16个微通道,并且由一个玻璃微型芯片和一个塑料外壳可以组成微槽和记录室。 该芯片可重复使用,并且用于降低“粘性化合物”的非特异性结合的可能,并通过降低低化合物消耗从而改善效率成本。
2 -带控制器和操纵杆的电动扫描平台
机动扫描台用于自动移动芯片位置。 它包括一个扫描台的控制器和一个操纵杆,并且可以由 Dynaflow® Commander 软件控制。
3 – 注射泵
配有一个易于安装的注射泵并且专门设计用于形成 Dynaflow® Resolve 芯片中的流柱。
4 – Dynaflow® Commander 软件
软件确保和控制扫描台的运动以及相关预编程。 它将精度的进行溶液交换与所获取实时数据以及进行标记同步。 基于 Windows 的 Dynaflow® Commander 软件可以quan面地掌控实验,包括扫描流程,给药时间和瞬时的实验流程改变。
文献:
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MEC-2 and MEC-6 in the Caenorhabditis elegans Sensory Mechanotransduction Complex: Auxiliary Subunits that Enable Channel Activity View paper
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Differential Structure of Atrial and Ventricular KATP Atrial KATP Channels Require SUR1 View paper
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Role of cAMP sensor Epac as a determinant of K-ATP channel ATP-sensitivity in human pancreatic beta cells and rat INS-1 cells View paper
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Gain-of-Function Mutations in the MEC-4 DEG/ENaC Sensory Mechanotransduction Channel Alter Gating and Drug Blockade View paper
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A Microfluidics Approach to the Problem of Creating Separate Solution Environments Accessible from Macroscopic Volumes View paper
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Automated Electrophysiology: High Throughput of Art View paper
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A Cell-Based Bar Code Reader for High-Throughput Screening of Ion Channel-Ligand Interactions View paper
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Screening of ion channel receptor agonists using capillary electrophoresis-patch clamp detection with resensitized detector cells View paper
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