单细胞应力加载系统,单个细胞机械力刺激培养系统,单个细胞应力加载,单细胞应力刺激培养

型号:cell confiner
联系人:李先生
联系电话:18618101725
品牌:法国cell confinement

单细胞应力加载系统,单个细胞机械力刺激培养系统,单个细胞应力加载,单细胞应力刺激培养


细胞被均匀地限制/压缩在两个亚微米分辨率的两个平行表面之间。
不同的限制高度(例如1um – 300um)
允许长期细胞培养和细胞增殖,同时保持对封闭的完美控制
与高分辨率光学显微镜系统兼容
可以处理足够多的细胞以进行完整的基因表达分析
可与生物功能化的微结构化底物和/或不同的基质(几何形状控制)结合使用
可以与凝胶结合(硬度控制)
兼容任何细胞培养底物(培养皿至96孔板)


产品:



应用:

Cell migration 2.5D, migration and interaction of non-adhesive cells, cell squeezing, imaging of flat cells (organelles aligned in 2D), super-resolution video-microscopy (organelles move less), contractility assay, etc
Confinement illustration
HeLa cells: not confined, 5 ?m, 3 ?m.
Explore examples of applications

> Cancer invasiveness assay: Quantification of migration behaviors and migration transitions
> Cancer aggressiveness assay: Quantification of contractility of somatic or cancer cells
> Endocytosis assay: Improved observation of events taking place at the membrane
> Exocytosis assay: Improved observation of events taking place at the apical membrane
> Frustrated phagocytosis: Characterization of the mechanism
> Immune system in a well: 2D migration and interaction of non-adherent immune cells
> Immune cells interaction: 2D interaction of non-adherent immune cells
> Mitotic assembly assay: Quantification of mitotic spindle disorders
> Quantitative cell migration assay: Fast and fine analysis of cell migration properties

文献:

PUBLICATIONS

 

  • Confinement and Low Adhesion Induce Fast Amoeboid Migration of Slow Mesenchymal Cells
    Y.-J. Liu, M. Piel, Cell, et al., 2015 160(4), 659-672
  • Actin flows induce a universal coupling between cell speed and cell persistence
    P. Maiuri, R. Voituriez, et al., Cell, 2015 161(2), 374–386
  • Geometric friction directs cell migration
    M. Le Berre, M. Piel, et al., Physical Review Letter 2013 111, 198101
  • Mitotic rounding alters cell geometry to ensure efficient spindle assembly
    O. M. Lancaster, B. Baum, et al., Developmental Cell, 2013 25(3), 270-283
  • Fine Control of Nuclear Confinement Identifies a Threshold Deformation leading to Lamina Rupture and Induction of Specific Genes
    M. Le Berre, J. Aubertin, M. Piel, Integrative Biology, 2012 4 (11), 1406-1414
  • Exploring the Function of Cell Shape and Size during Mitosis
    C. Cadart, H. K. Matthews, et al., Developmental Cell, 2014 29(2), 159-169
  • Methods for Two-Dimensional Cell Confinement
    M. Le Berre, M. Piel, et al., 2014, Micropatterning in Cell Biology Part C, Methods in cell biology, 121, 213-29

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